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Eco-Friendly Extraction of Flavonoids from Barley Waste Using Deep Eutectic Solvents for Sustainable Resource Utilization

Objective

Brewer's spent grain (BSG) is a significant by-product of beer brewing, accounting for 85% of the waste produced and 31% of the original malt weight. Therefore, there is a need to explore various recycling methods for this residue and transform it into value-added products. The objectives of this proposal align with several UN Sustainable Development Goals as they address sustainable food production, and sustainable consumption, focusing on health and well-being. In this study, we aim to utilize the bioactive phenolic compounds present in BSG to improve the shelf life of meat products. To achieve this, we will use environmentally and socioeconomically innovative methods for the valorization of BSG by using Deep eutectic solvents (DES) for the first time. The use of environmentally friendly solutions for the extraction of BSG flavonoids will present improvement in terms of cost, safety, and environmental friendliness compared to existing methods for extractraction. Optimization of extraction conditions (temperature, solvent-to-solid ratio, extraction time, pH) will be carried out to improve the selectivity, efficiency, and purity of target compounds. DES a solvent will be synthesized to selectively promote the extraction of rutin, proanthocyanidins, and catechin extract with phenolic acids. An important aspect of this work will be to evaluate the toxicity of both the DES solvent and the extracted compounds to ensure their safe use. A number of analytical techniques will be used to evaluate the extraction efficiency of the flavonoid compounds, primarily high-performance liquid chromatography (HPLC) in combination with mass spectrometry (MS). In addition, various methods will be carried out to evaluate the antioxidant profiles of the extracts. Finally, the proposed project will demonstrate the use of the extracts in red meat products (pork and beef) and evaluate how these extracts can positively influence the shelf life and sensory properties of such products.

Coordinator

KEMIJSKI INSTITUT
Net EU contribution
€ 155 559,36
Address
HAJDRIHOVA 19
1000 Ljubljana
Slovenia

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Region
Slovenija Zahodna Slovenija Osrednjeslovenska
Activity type
Research Organisations
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Total cost
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