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Effects of ALAN on lunar biology at sea: Characterization of sensory-neurosecretory cell type plasticity in adult Platynereis dumerilii from protected and light polluted areas

Project description

Unveiling the impact of light pollution on marine organisms

Increasing evidence indicates that artificial light at night (ALAN) disrupts the natural rhythms of marine organisms and affects reproduction. Recent findings in the worm Platynereis dumerilii show that certain brain cells in these worms change with the lunar cycle. However, the underlying mechanism of this phenomenon and how ALAN alters it remain unclear. With the support of the Marie Skłodowska-Curie Actions programme, the BrightPlaty project aims to understand how light pollution interferes with biological clocks at the cellular level. In this context, researchers will use single cell sequencing to map cell types involved in lunar rhythms and investigate the effects of ALAN.

Objective

In recent years, researchers have increasingly focused on the effects of artificial light at night (ALAN) polluIn recent years, researchers have increasingly focused on the effects of artificial light at night (ALAN) pollution on behavior and physiology of many terrestrial and marine organisms. ALAN particularly interferes with the circadian and circalunar rhythms, which are used to coordinate reproduction in many ecologically important marine organisms, such as brown algae, corals, and annelids. The bristle worm Platynereis dumerilii has been established as a leading model organism to characterize the process controlling circadian and circalunar rhythms. Recent studies by the Raible and Tessmar-Raible laboratories (Vienna, Austria) revealed the temporal plasticity of a specific cell cluster in the adult worm brain, which is thought to orchestrate lunar rhythms. However, this intriguing discovery was made under laboratory conditions, prompting further investigation into how this translates in wild animals and whether it is affected by ALAN. This project will be hosted in the Tessmar-Raible (TR) lab at the Max Perutz Labs (MPL) - University of Vienna (Univie), Austria, and it has three objectives: (1) to obtain a complete atlas of the cell types showing lunar plasticity in P. dumeriliis brain by performing the single-cell transcriptomic experiment over the course of two lunar cycles; (2) to characterize the cell type plasticity in wild P. dumerilii; (3) to identify the effects of ALAN on such response. These objectives represent significant advancements in the field, as the plasticity of cell types in response to lunar and circadian cycles is poorly understood and has been rarely explored using single-cell sequencing. Additionally, integrating data from wild specimens will allow for an unprecedented assessment of these processes in natural conditions. Exploring the influence of ALAN on single-cell plasticity also introduces a novel dimension to single-cell analysis.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2024-PF-01

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Coordinator

UNIVERSITAT WIEN
Net EU contribution

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€ 214 344,72
Address
UNIVERSITATSRING 1
1010 WIEN
Austria

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Region
Ostösterreich Wien Wien
Activity type
Higher or Secondary Education Establishments
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Total cost

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