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Effects of ALAN on lunar biology at sea: Characterization of sensory-neurosecretory cell type plasticity in adult Platynereis dumerilii from protected and light polluted areas

Descrizione del progetto

Svelare l’impatto dell’inquinamento luminoso sugli organismi marini

Sempre più prove indicano che la luce artificiale notturna altera i ritmi naturali degli organismi marini, ripercuotendosi sulla loro riproduzione. Recenti scoperte nel verme Platynereis dumerilii mettono in mostra che alcune cellule cerebrali di questi vermi cambiano con il ciclo lunare; ciononostante, il meccanismo alla base di questo fenomeno e il modo in cui ALAN lo altera sono tuttora poco chiari. Con il sostegno del programma di azioni Marie Skłodowska-Curie, il progetto BrightPlaty si propone di comprendere le modalità con cui l’inquinamento luminoso interferisce con gli orologi biologici a livello cellulare. In questo contesto, i ricercatori utilizzeranno il sequenziamento a singola cellula per mappare i tipi di cellule coinvolte nei ritmi lunari e studiare gli effetti generati dalla luce artificiale notturna.

Obiettivo

In recent years, researchers have increasingly focused on the effects of artificial light at night (ALAN) polluIn recent years, researchers have increasingly focused on the effects of artificial light at night (ALAN) pollution on behavior and physiology of many terrestrial and marine organisms. ALAN particularly interferes with the circadian and circalunar rhythms, which are used to coordinate reproduction in many ecologically important marine organisms, such as brown algae, corals, and annelids. The bristle worm Platynereis dumerilii has been established as a leading model organism to characterize the process controlling circadian and circalunar rhythms. Recent studies by the Raible and Tessmar-Raible laboratories (Vienna, Austria) revealed the temporal plasticity of a specific cell cluster in the adult worm brain, which is thought to orchestrate lunar rhythms. However, this intriguing discovery was made under laboratory conditions, prompting further investigation into how this translates in wild animals and whether it is affected by ALAN. This project will be hosted in the Tessmar-Raible (TR) lab at the Max Perutz Labs (MPL) - University of Vienna (Univie), Austria, and it has three objectives: (1) to obtain a complete atlas of the cell types showing lunar plasticity in P. dumeriliis brain by performing the single-cell transcriptomic experiment over the course of two lunar cycles; (2) to characterize the cell type plasticity in wild P. dumerilii; (3) to identify the effects of ALAN on such response. These objectives represent significant advancements in the field, as the plasticity of cell types in response to lunar and circadian cycles is poorly understood and has been rarely explored using single-cell sequencing. Additionally, integrating data from wild specimens will allow for an unprecedented assessment of these processes in natural conditions. Exploring the influence of ALAN on single-cell plasticity also introduces a novel dimension to single-cell analysis.

Campo scientifico (EuroSciVoc)

Parole chiave

Programma(i)

Argomento(i)

Meccanismo di finanziamento

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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(si apre in una nuova finestra) HORIZON-MSCA-2024-PF-01

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Coordinatore

UNIVERSITAT WIEN
Contributo netto dell'UE
€ 214 344,72
Indirizzo
UNIVERSITATSRING 1
1010 Wien
Austria
Regione
Ostösterreich Wien Wien
Tipo di attività
Higher or Secondary Education Establishments
Collegamenti
Costo totale
Nessun dato