Objective
During differentiation, dramatic changes occur in the biochemical processes and the architecture of cells. The subcellular localization of proteins greatly impacts their function and also the transformations during cell differentiation are accompanied or facilitated by protein translocation. In the proposed research project, I want to systematically study how proteins translocate during the differentiation from neuronal stem cells into neurons and astrocytes. To this end, I aim to develop a general and efficient screening method for protein translocation during cell differentiation. I envision to identify protein translocation behavior that is essential for neurodevelopment and direct reprogramming, which I plan to confirm in brain organoids, which include diverse cell types. Additionally, I want to study the underlying mechanisms both initiating and initiated by protein translocation.
The method will be based on fluorescence microscopy, which will allow me to observe the dynamics of protein localization in living, differentiating cells. To increase the efficiency of the screening approach, I want to implement a recently published multiplexing strategy, temporally multiplexed imaging (TMI). This method uses the photo-switching kinetics of the utilized fluorescent protein (FP) labels to deconvolute up to 6 signals in a single spectral channel. In this way, I can label several proteins per cell multiplying the efficiency of the method and enabling the screening of a large panel of proteins. I will label these proteins with TMI-compatible FPs through gene editing. Imaging of the edited cells throughout the differentiation process and subsequent automated image analysis will identify translocating proteins. The gained mechanistic knowledge about neuronal cell differentiation will improve our basic understanding of this process eventually facilitating the development of improved disease models as well as medical applications such as reprogramming.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-GF - HORIZON TMA MSCA Postdoctoral Fellowships - Global Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2024-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
85764 Neuherberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.