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VIVA-ELISA: Advanced Laboratory Test for Rapid Detection of NLRP3 Inflammasome Activation in Critical Septic Patients

Objective

Sepsis causes almost 20% of global annual deaths. It involves a dysregulated systemic inflammatory response driven by proinflammatory cytokines. If untreated, it progresses to organ dysfunction, low blood pressure, and septic shock. Current diagnosis methods have low predictability, delays in diagnosis, lack of sensitivity, and an inability to predict the course of the patients deterioration: there is thus a need for a rapid, accurate, affordable and early identification of the mortality risk.
VIVA-ELISA is a follow-up to the ERC project SPEDI-TEST conducted at the Biomedical Research Institute of Murcia (IMIB), of which Viva in Vitro is a spin-off. Studies show that 100% of deaths of septic patients are correlated to early impairment of the NLRP3 inflammasome activation in monocytes, in the initial 24 h. A novel in vitro diagnostic method was developed for the fast detection of early impairment of the NLRP3 inflammasome activation. This method can stratify patients at a higher risk of developing life-threatening complications, resulting in an early and more accurate prognosis.
VIVA-ELISA will advance this concept by developing an easy-to-use, highly sensitive immunoassay that can be seamlessly implemented into hospitals and clinics, reducing time-to-treatment, variability in data interpretation, and hospitalization costs, currently averaging $32,000 per patient. Our solution, designed as a classic quantitative sandwich ELISA, requires only a small blood sample to measure ex vivo NLRP3 activation. We aim to reduce sepsis mortality rates by 25 to 40%, reduce morbidity and recovery time, reduce chronic sequelae for patients and enable the possibility of personalized treatments and immunotherapy, while also contributing to the sustainability of healthcare systems.
VIVA-ELISA will also validate this technology in initial clinical studies and will carry out the necessary work to comply with current EU/US regulations before its introduction into the market.

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HORIZON-EIC - HORIZON EIC Grants

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Call for proposal

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(opens in new window) HORIZON-EIC-2024-TRANSITION-01

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Coordinator

VIVA IN VITRO DIAGNOSTICS SL
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 493 003,89
Address
PQUE CIENTIFICO MURCIA CTRA DE MADRID KM 388 EDIF T PLANTA 1
30100 MURCIA
Spain

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Sur Región de Murcia Murcia
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
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Total cost

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