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Revolutionary OnLine Fermentation monitoring

Project description

Holographic microscopy and AI support real-time fermentation monitoring

Producing food through fermentation and microalgae holds enormous promise to decouple production from traditional land-intensive agriculture. Without proper real-time monitoring and control of the microorganisms and processes, production has struggled to scale beyond laboratory settings. The EIC-funded ROLF project aims to solve this by developing the world’s most advanced bioprocess monitoring and control platform. Using cutting-edge holographic microscopy (a laser-based imaging technique) combined with AI, it will analyse millions of microorganisms in minutes, detecting problems early and enabling process optimisation in real time. Delivered as an easy-to-integrate, software-as-a-service and sensor package, ROLF’s platform could double output while using fewer resources, making sustainable, soil-free food production viable at a global scale.

Objective

Holloid will transform food production based on precision fermentation and microalgae cultures with the world’s most advanced real-time bioprocess monitoring and control platform. The current absence of real-time monitoring systems capable of characterizing different microorganisms and cells in bioprocesses, including their states, hinders breakthroughs in bioprocess production of food ingredients from transitioning beyond the lab, greatly reduces productivity, and leads to frequent waste. Primarily, companies in this area face challenges in 1) quickly detecting low concentrations of spoiler microorganisms to avoid waste and 2) monitoring all microorganisms in large liquid volumes to develop and control new and optimized bioprocesses.
Holloid develops a unique bioprocess control system based on groundbreaking high-throughput, automated in-line holographic microscopy. In this project, we will add pre-emptive remote maintenance, upscale secure data management pipelines, and use AI to distinguish microorganisms and their phenotypic states in real-time. Our platform will analyze millions of microorganisms in minutes, identifying even rare populations with high statistical significance. It is the missing key enabling technology, giving food bioprocess engineers the ability to directly monitor the microorganisms present and control production with unprecedented precision. We will deliver actionable insights in real-time that our pilot customers say can double their output while using significantly fewer resources, critically enhancing the quality and safety of fermentation and microalgae products. Our scalable SaaS platform and low-cost sensors will be easily integrated into any production process, making statistically relevant, single-cell monitoring accessible to businesses everywhere via a user-friendly web interface.
Holloid aims to become an integral part of the production value chain for bioprocesses that decouple food production from soil and the environment.

Keywords

Programme(s)

Topic(s)

Funding Scheme

HORIZON-EIC-ACC - HORIZON EIC Accelerator

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Call for proposal

(opens in new window) HORIZON-EIC-2024-ACCELERATOR-02

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Coordinator

HOLLOID GMBH
Net EU contribution
€ 2 499 999,00
Address
MUTHGASSE11/2/1
1190 WIEN
Austria
SME
Yes
Region
Ostösterreich Wien Wien
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Links
Total cost
No data