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Positron Annihilation Lifetime Spectroscopy for Revealing and Quantifying Inflammation and Endothelial Diseases

Project description

New tool to detect inflammatory diseases

Inflammation-related diseases often involve tiny changes in blood vessel walls that are hard to detect with current tools. The ERC-funded ALFRED project will address this using positron annihilation spectroscopy (PAS) – a technique that examines molecular changes at incredibly small scales without damaging the tissue. Researchers will create a model of blood vessel walls and develop a special device to test how these walls respond to different conditions. PAS will be combined with conventional techniques to offer a clearer picture of how inflammation affects blood vessels. The proposed approach could lead to earlier detection of diseases, improving treatment options and patient outcomes.

Objective

Inflammatory processes have been identified as major contributors to various mental and physical diseases, significantly impacting global morbidity and mortality. Many of these conditions involve changes in the microvascular permeability that are challenging to early detect due to limitations in current diagnostic tools, which are either invasive or lack sufficient resolution. There is an urgent need for improved methods to detect and measure microvascular permeability. To advance the field, I will use Positron Annihilation Spectroscopy (PAS) to quantify alterations in the capillary endothelial wall integrity directly, known to be correlated to the endothelial permeability. PAS is a non-destructive, label-free technique that measures molecular dynamics at the nanoscale. By irradiating materials with positrons and analyzing their resulting annihilation events, PAS can provide real-time observations of nanostructural changes in biological tissue.
To achieve my goal, I will engineer a robust capillary endothelial wall model and I will characterize endothelial wall permeability changes under various pharmacological conditions, by using electrophysiological techniques. To perform intravital PAS on this in vitro model, I will develop a novel perfusion bioreactor, able to withstand high vacuum conditions and to interface biological matter with positron sources. I will then correlate PAS outputs with the ones of conventional techniques. To optimize drug concentration required to measure PAS variations, I will realize a numerical predictive model. Finally, I will validate my approach on the highly vascularized respiratory membrane of embryonated avian eggs. This project combines bioengineering, particle physics, and medical imaging to address a critical gap in current diagnostic capabilities, revolutionizing preventive diagnostics, and offering patients a new tool for early detection of inflammatory and endothelial diseases, that could improve their life expectancy.

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HORIZON-ERC - HORIZON ERC Grants

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(opens in new window) ERC-2025-STG

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Host institution

POLITECNICO DI MILANO
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 499 901,00
Address
PIAZZA LEONARDO DA VINCI 32
20133 Milano
Italy

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Region
Nord-Ovest Lombardia Milano
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 499 901,00

Beneficiaries (1)

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