Objective
I propose a chemoproteomic platform for the discovery of unchartered eraser activities regulating post-translational modifications within the ubiquitin system. This approach will be enabled by a suite of advanced ubiquitin probes. These reagents recapitulate the complexity of cellular polyubiquitin modifications and are equipped with novel electrophilic handles to report on polyubiquitin chain length- and linkage-dependent activities of deubiquitinating enzymes. These enzymes reverse ubiquitin modifications and critically determine the outcome of ubiquitin-mediated signalling, yet how they decode ubiquitin chains has remained poorly understood. Moreover, activity-based probes with novel designs will enable chemoproteomic experiments to identify enzymes with novel catalytic mechanisms and with relevance in cellular stress response processes. These insights will subsequently reveal molecular mechanisms of chain-length decoding and substrate recognition by facilitating the structural analysis of DUB-probe complexes. Taken together, the proposed work breaks new ground through a rational workflow for comprehensively mining the proteome for unprecedented DUB/Ubl eraser activities. Expected results will have broad implications also for ubiquitin assembly enzymes, pathogen-derived virulence factors and other protein classes. UbiPRO will thus illuminate a fundamentally important and evolutionarily conserved system of molecular information encoding employed by all forms of life.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2025-COG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
45141 ESSEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.