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Plasmonic nanopore technologies for automatic manipulation and Raman spectroscopic sequencing of single proteins

Objective

The human proteome offers unprecedented insights into health, diseases and treatment. The current protein detection techniques are either expensive, insensitive, complex or introduce biases. Meanwhile, no amplification methods for proteins exist, which complicates their detection and leads to a serious lag of proteomics behind genomics and transcriptomics, hampering not only mechanistic studies but also clinical applications. The RamanProSeq project aims to develop a label-free optical single-molecule protein sequencing technology capable of reading protein primary sequence at single-amino-acid resolution with high accuracy (>99.9%). The proposed technology will revolutionise the field of proteomics towards personalized medicine and early diagnosis of diseases. The project will be based on the partners' approaches: ultrafast Raman spectroscopy with 1 microsecond time resolution and plasmonic nanopore-enhanced Raman sensors that have demonstrated single-molecule identification of 20 proteogenic amino acids, their post-translational modifications and discrimination of single amino acid residues within single peptides. Such achievement has addressed the long-standing challenge of current single-molecule technologies that cannot detect all 20 proteinogenic amino acids. Based on these approaches, the RamanProSeq project will integrate solid-state nanopores with a high-speed Raman detection system, an automated control system, computer simulations, and advanced Raman-based bioinformatics. The output of the project will be a novel nanopore-based device that will be used to sequence individual proteins at low concentrations and with high accuracy. The project will enable low cost, high resolution and high throughput in single-protein sequencing for a broad range of applications in life science and beyond.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-EIC - HORIZON EIC Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-EIC-2025-PATHFINDEROPEN

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Coordinator

OULUN YLIOPISTO
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 743 903,75
Address
PENTTI KAITERAN KATU 1
90014 Oulu
Finland

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Region
Manner-Suomi Pohjois- ja Itä-Suomi Pohjois-Pohjanmaa
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 743 903,75

Participants (5)

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