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Enzyme-integrated high-throughput Multicomponent synthesis for Echo-enabled Rapid molecule generation and Assay in Lead Discovery

Objective

Indoles are among the most widely encountered scaffolds in medicines and natural products, yet general strategies for their systematic diversification remain limited. Through EMERALD (Enzyme-integrated high-throughput Multicomponent synthesis for Echo enabled Rapid molecule generation and Assay in Lead Discovery), I will unites three complementary techniques into the first miniaturized, automated pipeline for late-stage indole diversification at nanoliter scale (i) multicomponent reactions for efficient and modular assembly of indole frameworks; (ii) enzymatic tailoring, enabling regio- and chemoselective functionalization; and (iii) acoustic droplet ejection combined with real-time mass spectrometry, delivering ultra-high throughput synthesis and analytics with >90% reductions in solvent use. During a secondment at the University of St Andrews, I will evaluate enzyme panels to produce compatibility and selectivity maps directly relevant to drug discovery. Importantly, a subset of the resulting functionalized indoles will be screened against the p53–MDM2 interaction, a clinically validated target in oncology, to demonstrate the platforms’ direct translational potential. By marrying chemical and enzymatic methodologies, EMERALD establishes a sustainable route to novel scaffolds and natural product–inspired analogues, while providing an ideal interdisciplinary training ground at the interface of chemistry, biocatalysis, and automation-ultimately advancing greener drug discovery and fostering my path to research independence.

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Topic(s)

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2025-PF

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Coordinator

UNIVERZITA PALACKEHO V OLOMOUCI
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 191 918,16
Address
KRIZKOVSKEHO 8
771 47 Olomouc
Czechia

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Region
Česko Střední Morava Olomoucký kraj
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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