Objective
Sustained proliferation is a hallmark of cancer cells, frequently driven by alterations in proto-oncogenes (e.g. HRAS or MYC). While oncogene activation increases the cellular proliferation rate, it also triggers cellular stress responses. This dynamic creates a delicate equilibrium between continued cell growth and cell cycle arrest, making oncogene activation a powerful model for studying divergent cell fate decisions at the single-cell level. Despite extensive studies, the phenotypic traits that drive this equilibrium toward proliferation or arrest remain poorly understood. In this project, I propose to study the phenotypic heterogeneity of oncogene activation using ‘IRIS’ (Bues et al., in preparation), a novel single-cell phenomics approach developed by my host lab. IRIS enables the simultaneous acquisition of high-resolution images and transcriptomic profiles from individual cells at a high throughput (>1,000 cells/h). Using this technology, I aim to test our hypothesis that the dosage of c-Myc oncoprotein and the heterogeneity of HRASV12 subcellular localization modulate cell fate decisions through differential activation of biosynthetic and stress-response pathways. Specifically, I will (1) identify phenotypic determinants modulating oncogene-induced heterogeneity, (2) develop a screening platform to map morphological-to-transcriptomic changes associated with oncogene activation, and (3) functionally characterize the phenotypic traits associated with oncogene-indicated heterogeneity. By linking image-based traits to functional cell states through transcriptome analysis, I aim to uncover molecular determinants of cell fate in response to oncogenic stress. This work will provide fundamental insight into heterogeneity of early stages of cancer, and also reveal phenotypes with long-term translational potential for cancer diagnostics.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics RNA transcriptomes
- medical and health sciences clinical medicine oncology
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2025-PF
See all projects funded under this callCoordinator
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1015 LAUSANNE
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.