Objective
The overall aim of the fellowship is to analyse the molecular evolution of a family of genes in fungi, which cause powdery mildew diseases of plants. Avirulence genes elicit host defences in gene-for-gene interactions with plants. The family to be studied is related to the Avrk1 gene in the barley mildew, Blumeria graminis f.sp. hordei.
The specific objectives are:
1. To research the evolution of members of the Avrk1-like gene family in diverse isolates of B. graminis hordei. This will identify elements of the genes, which are:
(a) conserved and may be required for pathogenicity,
(b) subject to diversifying selection, so probably involved in gene-for-gene interactions,
(c) not essential for pathogenicity. The work will involve culturing of B. graminis isolate s, cloning Avrk1-like fragments by homology with Avrk1, and sequencing of Avrk1-like genes, followed by phylogenetic analysis of sequences.
2. To examine host-specialisation of wheat and barley mildew by investigating the divergence of members of the Avrk1 -like family, and to research the distribution of Avrk1-like genes in diverse mildew pathogens of dicot plants. The methods used will be similar to those in Objective 1.
3. To analyse the association of Avrk1-like genes with avirulences, to identify Avrk1-like sequences as candidate avirulence genes. This will involve crossing B. graminis isolates, extracting ascospore progeny, and analysis of avirulence on a differential set of barley varieties. Avrk1 homologues will be analysed for sequence variation between the parents of the cross and polymorphisms mapped in the progeny to investigate their linkage to avirulence loci.
The fellowship will provide Ms Sacristan with expert training in:
1. Phylogenetic methods applied to evolution of DNA sequences (Objective s 1 and 2);
2. Fungal genetics and genetic analysis (Objective 3);
3. Advanced methods of molecular marker analysis (Objectives 1 and 3);
4. Fungal plant pathology and mycological methods (all 3 Objectives).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences microbiology mycology
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences molecular biology molecular evolution
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NORWICH
United Kingdom
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