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Content archived on 2024-05-29

Challenging the hidden HIV: understanding the block on transcriptional reactivation to eradicate infection

Objective

Highly active antiretroviral therapy (HAART) has profoundly decreased morbidity and mortality in human immunodeficiency virus type 1 (HIV-1)-infected individuals. Nonetheless, HIV-1 has not been eradicated by HAART. Factors affecting HIV-1 latency presen t formidable obstacles for therapeutic intervention and the need for novel therapeutic strategies specifically targeting HIV-1 latency has become evident. However, therapeutic targeting of HIV-1 latency requires an understanding of the basic mechanisms r egulating viral quiescence and activation. Exploring the scientific possibilities of new therapies targeting HIV-1 latency may hold new promise of eventual HIV-1 eradication. The proposal aims at identifying the key cellular and viral factors that control HIV latency and reactivation. Cellular factors that affect HIV-1 latency could be proteins controlling chromatin silencing and transcriptional reactivation through interaction with viral proteins, as well as small interfering RNAs produced naturally in t he infected cells that control silencing. Furthermore, we intend to explore the possibility that HIV-1 itself could modulate the cellular siRNA machinery to control its own expression. In order to approach these topics a wide array of techniques will be implemented. A Proteomic approach will be used to identify cellular factors involved in the control of viral latency and reactivation. The recruitment of these factors to the integrated viral promoter will be monitored by chromatin immunoprecipitation a nd by highly innovative fluorescent optical tools. Finally, the information gained from these basic studies will be exploited to design specific siRNA targeted to viral latency.

Fields of science (EuroSciVoc)

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Keywords

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

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FP6-2003-LIFESCIHEALTH-3
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Funding Scheme

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STREP - Specific Targeted Research Project

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
EU contribution
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Total cost

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Participants (4)

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