During the project, we have performed the following activities related to the product development:
• Designed and validated a high‑yield, chemolithoautotrophic fermentation using non‑pathogenic gram‑negative strains to convert captured industrial CO2 (with H2) directly into PHA; established process parameters and redox pathway activation for autotrophic metabolism.
• Built and operated 10 L bioreactor prototypes producing PHA from industrial‑grade CO2; optimized yields and downstream extraction; verified compostability (ASTM D6400/D6868) and marine biodegradability.
• Conducted material characterization and performance testing; demonstrated <10% deviation in key thermal transitions and textile stability through laundering and ironing.
• Developed a non‑toxic solvent extraction route that outputs filament‑ready PHA fibres, reducing processing costs; integrated fibre‑spinning concepts for pilot in‑line production.
• Collected parameters scale‑up engineering for a 10,000 L pilot (~3 t/year) and prepared field testing at Infraserv Industriepark Höchst, including fibre‑spinning integration.
• Completed feasibility and demonstrations with SABIOMATERIALS, producing first consumer articles (socks, baskets) from CO2‑PHA to validate end‑to‑end continuity.
• Advanced IP and freedom to operate filed European patent, conducted FTO assessment, secured trademark, and progressed business/risk plans for industrial deployment.
Our main achievements reached during the project include:
• Proven prototype conversion efficiency: up to ~1 kg PHA from ~2.5 kg CO2, validating bioprocess scalability, strain selection, and process control for consistent quality.
• Validated material performance and end-user viability: CO2‑PHA matches reference thermal/mechanical properties (DSC/TGA/ISO 527; <10% deviation), is compostable per ASTM D6400/D6868, marine biodegradable, and maintains textile durability after 50 wash cycles at 40°C and ironing, supporting substitution in fibre applications.
• Established tuneable PHA composition and fibre diameters (~1–100 µm) enabling different applications and reducing the downstream costs. Ou top applications include biodegradable textiles (monofilaments and multifilaments), films for packaging, tree shelters and ingredients for cosmetics.
• Achieved integrated industrial design: on‑site CO2 capture and immediate bioconversion create a circular route, avoid atmospheric release, and enable EUA cost offsets while producing saleable feedstock.
• Secured pilot implementation pathway with partners to finalize parameters for first commercial‑scale site, including fibre‑spinning for direct distribution.
• Our pilot plan started operations in summer 2024. The cumulated produced PHA (including third party PHA) reached the 2 Kg (~2,21 tons).