Objective
Networks of protein-protein interactions are central to cell and developmental biology and of great importance in molecular medicine. Given the potential importance of protein-protein interactions to healthcare, wealth creation and the biotech industry the re is an urgent need to train young researchers in the latest technologies aimed at identifying and dissecting such interactions by the very latest in high-throughput approaches, bio-informatics and rigorous biophysical analysis. We will train early-stage and experienced researchers to use new bio-informatics tools to direct high-throughput screening (HTS) for new protein-protein interactions. Network "hubs" or "nodes" (i.e. proteins/protein families that interact crucially with one or many other partners in the network) will be identified and used as baits in HTS.
Based on previous collaborative, published work, we will develop an in vitro selection system for protein-protein interactions by in vitro compartmentalisation (IVC). IVC relies on dispersing aqueous reactions into microscopic water-in-oil emulsion droplets ( ~5 fl) enabling selection of 10e10 pairs of interacting proteins. Hence, all ~10e9 possible pair wise binding interaction of proteins encoded by the human genome are possible in a more economical format than existing HTS techniques.
A novel system for covalently labelling proteins will be used to label and cross-link proteins in vivo and to create protein micro-arrays. Kinetic and thermodynamic characterisation of identified protein-protein interact ions will be performed using a variety of techniques including a novel acoustic biosensor. Most importantly, we aim at an integrated approach where the bio-informatics tools we develop will be used to direct the HTS efforts. In turn, results obtained from t he screening will help validate and tune our newly-developed computational tools, while detailed mechanistic studies will provide insight into the physiological role of newly-identified interacting pairs.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences developmental biology
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-1
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CAMBRIDGE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.