Objective Tuberculosis (TB) continues being a leading cause of death due to a single infectious disease agent. The HIV/AIDS pandemic and the emergence of drug resistance are compounding factors that hinder the control of the disease. Associated with this problem is the emergence of multidrug-resistant (MDR) strains of Mycobacterium tuberculosis, defined as strains resistant to at least isoniazid and rifampicin, the most valuable drugs in the treatment of the disease. More recently, the appearance of extensively drug resistant (XDR) strains has been reported. These strains, in addition to being MDR, are also resistant to key second-line drugs. Patients, especially HIV patients, harbouring XDR strains have virtually no treatment options. New and improved methods for fast detection of drug resistance are urgently needed. This project will develop a twofold-approach system for the fast and simultaneous detection of MDR and XDR strains based on a rapid phenotypic assay and a genotypic test. Colorimetric methods, which have been previously validated by our group for first-line drug susceptibility testing, will be developed for key second-line drugs involved in XDRTB. Once set up, these methods will be further elaborated for direct application to sputum specimens. The molecular tool will be based on a modification of the novel technology named detection of immobilized amplified product in one phase system. This single step amplification method will be developed in a versatile microtitre well strip format for detection first of MDR and then of XDR strains. The tools will be then validated in different settings and prospectively evaluated in target populations. The project will contribute to the currently available armamentarium for rapid detection of drug resistant TB and will introduce new tools for the detection of the recently-described and highly-lethal XDRTB. It will also contribute to our knowledge on the mechanisms of M. tuberculosis resistance to second-line drugs. Fields of science medical and health scienceshealth sciencesinfectious diseasesRNA virusesHIVmedical and health sciencesclinical medicinepneumologytuberculosismedical and health sciencesbasic medicinepharmacology and pharmacydrug resistancemultidrug resistance Keywords MDR XDR drug resistance fast detection new tests tuberculosis Programme(s) FP7-HEALTH - Specific Programme "Cooperation": Health Topic(s) HEALTH-2007-2.3.2-3 - Development of fast tests for the diagnosis of Multi-Drug-Resistant strains of HIV, malaria and tuberculosis and of latent tuberculosis infection (LTBI) Call for proposal FP7-HEALTH-2007-A See other projects for this call Funding Scheme CP-FP - Small or medium-scale focused research project Coordinator INSTITUUT VOOR TROPISCHE GENEESKUNDE Address Nationalestraat 155 2000 Antwerpen Belgium See on map Region Vlaams Gewest Prov. Antwerpen Arr. Antwerpen Activity type Higher or Secondary Education Establishments Administrative Contact Jef Van Lint (Mr.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution € 868 985,00 Participants (7) Sort alphabetically Sort by EU Contribution Expand all Collapse all HELMHOLTZ-ZENTRUM FUR INFEKTIONSFORSCHUNG GMBH Germany EU contribution € 336 000,00 Address Inhoffenstrasse 7 38124 Braunschweig See on map Region Niedersachsen Braunschweig Braunschweig, Kreisfreie Stadt Activity type Research Organisations Administrative Contact Michael Strätz (Dr.) Links Contact the organisation Opens in new window Website Opens in new window SMITTSKYDDSINSTITUTET Sweden EU contribution € 420 000,00 Address Nobels vaeg 18 17182 Solna See on map Activity type Research Organisations Administrative Contact Bengt-åke Andersson (Mr.) Links Contact the organisation Opens in new window Website Opens in new window LIONEX GMBH Germany EU contribution € 330 300,00 Address Salzdahlumer strasse 196 38126 Braunschweig See on map Region Niedersachsen Braunschweig Braunschweig, Kreisfreie Stadt Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Mahavir Singh (Prof.) Links Contact the organisation Opens in new window Website Opens in new window LATVIJAS INFEKTOLOGIJAS CENTRS (INFECTOLOGY CENTER OF LATVIA) Latvia EU contribution € 125 200,00 Address Linezera iela 3 1006 Riga See on map Activity type Research Organisations Administrative Contact Girts Skenders (Dr.) Links Contact the organisation Opens in new window Website Opens in new window CORPORACION CORPOGEN Colombia EU contribution € 352 200,00 Address Carrera 5 n° 66a-34 N/A Bogota See on map Activity type Research Organisations Administrative Contact Patricia Del Portillo (Ms.) Links Contact the organisation Opens in new window Website Opens in new window ADMINISTRACION NACIONAL DE LABORATORIOS E INSTITUTOS DE SALUD DR. CARLOS G. MALBRAN Argentina EU contribution € 131 200,00 Address Avenida velez sarsfield 563 1281 Buenos aires See on map Activity type Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Administrative Contact Laura Boveri (Ms.) Links Contact the organisation Opens in new window Website Opens in new window HOSPITAL ZONAL ESPECIALIZADO DE AGUDOS Y CRONICOS DR. CETRANGOLO Argentina EU contribution € 131 680,00 Address Italia 1750 1602 Vicente lopez See on map Activity type Research Organisations Administrative Contact Laura Boveris (Ms.) Links Contact the organisation Opens in new window Website Opens in new window
