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Development of a two-approach plate system for the fast and simultaneous detection of MDR and XDR M. tuberculosis

Objective

Tuberculosis (TB) continues being a leading cause of death due to a single infectious disease agent. The HIV/AIDS pandemic and the emergence of drug resistance are compounding factors that hinder the control of the disease. Associated with this problem is the emergence of multidrug-resistant (MDR) strains of Mycobacterium tuberculosis, defined as strains resistant to at least isoniazid and rifampicin, the most valuable drugs in the treatment of the disease. More recently, the appearance of extensively drug resistant (XDR) strains has been reported. These strains, in addition to being MDR, are also resistant to key second-line drugs. Patients, especially HIV patients, harbouring XDR strains have virtually no treatment options. New and improved methods for fast detection of drug resistance are urgently needed. This project will develop a twofold-approach system for the fast and simultaneous detection of MDR and XDR strains based on a rapid phenotypic assay and a genotypic test. Colorimetric methods, which have been previously validated by our group for first-line drug susceptibility testing, will be developed for key second-line drugs involved in XDRTB. Once set up, these methods will be further elaborated for direct application to sputum specimens. The molecular tool will be based on a modification of the novel technology named detection of immobilized amplified product in one phase system. This single step amplification method will be developed in a versatile microtitre well strip format for detection first of MDR and then of XDR strains. The tools will be then validated in different settings and prospectively evaluated in target populations. The project will contribute to the currently available armamentarium for rapid detection of drug resistant TB and will introduce new tools for the detection of the recently-described and highly-lethal XDRTB. It will also contribute to our knowledge on the mechanisms of M. tuberculosis resistance to second-line drugs.

Field of science

  • /medical and health sciences/basic medicine/pharmacology and pharmacy/drug resistance
  • /medical and health sciences/basic medicine/pharmacology and pharmacy/drug resistance/multidrug resistance
  • /medical and health sciences/clinical medicine/pneumology/tuberculosis

Call for proposal

FP7-HEALTH-2007-A
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

PRINS LEOPOLD INSTITUUT VOOR TROPISCHE GENEESKUNDE
Address
Nationalestraat 155
2000 Antwerpen
Belgium
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 868 985
Administrative Contact
Jef Van Lint (Mr.)

Participants (7)

HELMHOLTZ-ZENTRUM FUR INFEKTIONSFORSCHUNG GMBH
Germany
EU contribution
€ 336 000
Address
Inhoffenstrasse 7
38124 Braunschweig
Activity type
Research Organisations
Administrative Contact
Michael Strätz (Dr.)
SMITTSKYDDSINSTITUTET
Sweden
EU contribution
€ 420 000
Address
Nobels Vaeg 18
17182 Solna
Activity type
Research Organisations
Administrative Contact
Bengt-åke Andersson (Mr.)
LIONEX GMBH
Germany
EU contribution
€ 330 300
Address
Salzdahlumer Strasse 196
38126 Braunschweig
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Mahavir Singh (Prof.)
LATVIJAS INFEKTOLOGIJAS CENTRS (INFECTOLOGY CENTER OF LATVIA)
Latvia
EU contribution
€ 125 200
Address
Linezera Iela 3
1006 Riga
Activity type
Research Organisations
Administrative Contact
Girts Skenders (Dr.)
CORPORACION CORPOGEN
Colombia
EU contribution
€ 352 200
Address
Carrera 5 N° 66A-34
N/A Bogota
Activity type
Research Organisations
Administrative Contact
Patricia Del Portillo (Ms.)
ADMINISTRACION NACIONAL DE LABORATORIOS E INSTITUTOS DE SALUD DR. CARLOS G. MALBRAN
Argentina
EU contribution
€ 131 200
Address
Avenida Velez Sarsfield 563
1281 Buenos Aires
Activity type
Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments)
Administrative Contact
Laura Boveri (Ms.)
HOSPITAL ZONAL ESPECIALIZADO DE AGUDOS Y CRONICOS DR. CETRANGOLO
Argentina
EU contribution
€ 131 680
Address
Italia 1750
1602 Vicente Lopez
Activity type
Research Organisations
Administrative Contact
Laura Boveris (Ms.)