Objective This proposal aims at developing a versatile fermentation platform for the conversion of lipid feed stocks into diverse added-value products. It is proposed to develop the oleaginous yeast Yarrowia lipolytica into a microbial factory by directing its versatile lipid metabolism towards the production of industrially valuable compounds like wax esters (WE), polyhydroxyalkanoates (PHA’s), free hydroxyl fatty acids (HFA’s) and isoprenoid-derived compounds (carotenoids, polyenic carotenoid ester). Conversion of lipid intermediates into these products will be achieved by introducing heterologous enzyme functions isolated from marine hydrocarbonoclastic bacteria into Yarrowia. To achieve these goals we have assembled a team with a broad set of complementary expertise in microbial physiology, metabolic engineering, yeast lipid metabolism, metagenomics, biochemical and protein engineering. Already available for this project are a number of genetically engineered Yarrowia strains as well as a collection of genes encoding enzymes for the production of WE’s, 3-HFA’s, PHA’s and carotenoids. The following complementary research focus areas are proposed: (1) Engineering of metabolic precursor pools in Yarrowia lipolytica for the production of added-value products from lipids (INRA, UGe). (2) Conversion of metabolic precursor pools in Yarrowia to added-value products by overexpressing heterologous biosynthetic enzymes (UGe, INRA, UoM). (3) Discovery and characterization of novel aliphatic enzyme activities by metagenomic screening of marine hydrocarbonoclastic and other oil- and fat-metabolizing microbial communities (TUBS, UoN). The project is further complemented by: (i) the activity of a professional valorization company (Ascenion) providing IP protection and commercialization services; (ii) by proactive efforts to expand the project’s target products’ application potential (Avecom). Fields of science engineering and technologyindustrial biotechnologymetabolic engineeringnatural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsengineering and technologyindustrial biotechnologybiomaterialsbioplasticspolyhydroxyalkanoatesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Keywords Yarrowia lipolytica carotenoids hydrocarbonoclastic bacteria hydroxyl fatty acids isoprenoids lipids polyhydroxyalkanoates wax esters yeast Programme(s) FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Biotechnology Topic(s) KBBE-2007-3-2-02 - LIPID ENZYMES - Development of enzymes for lipid modification and activation Call for proposal FP7-KBBE-2007-1 See other projects for this call Funding Scheme CP-FP - Small or medium-scale focused research project Coordinator UNIVERSITEIT GENT EU contribution € 237 489,00 Address SINT PIETERSNIEUWSTRAAT 25 9000 Gent Belgium See on map Region Vlaams Gewest Prov. Oost-Vlaanderen Arr. Gent Activity type Higher or Secondary Education Establishments Administrative Contact Wim Soetaert (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (6) Sort alphabetically Sort by EU Contribution Expand all Collapse all INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT France EU contribution € 171 384,00 Address 147 RUE DE L'UNIVERSITE 75007 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Administrative Contact Pierre Paris (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data TECHNISCHE UNIVERSITAET BRAUNSCHWEIG Germany EU contribution € 162 238,00 Address UNIVERSITAETSPLATZ 2 38106 Braunschweig See on map Region Niedersachsen Braunschweig Braunschweig, Kreisfreie Stadt Activity type Higher or Secondary Education Establishments Administrative Contact Kenneth Timmis (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITY OF NAIROBI Kenya EU contribution € 120 000,00 Address University Way 00100 Nairobi See on map Activity type Higher or Secondary Education Establishments Administrative Contact Francis Mulaa (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data REGENTS OF UNIVERSITY OF MINNESOTA United States EU contribution € 120 000,00 Address OAK STREET SE 200 MCNAMARA ALUMNI CENTER 450 55455 2070 Minneapolis Mn See on map Activity type Higher or Secondary Education Establishments Administrative Contact Claudia Schmidt-Dannert (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data AVECOM Belgium EU contribution € 60 000,00 Address INDUSTRIEWEG 122P 9032 Gent-Wondelgem See on map Region Vlaams Gewest Prov. Oost-Vlaanderen Arr. Gent Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Wim De Windt (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data ASCENION GmbH Germany EU contribution € 40 000,00 Address Herzogstrasse 64 80803 Munich See on map Region Bayern Oberbayern München, Kreisfreie Stadt Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Sabina Heim (Dr.) Links Contact the organisation Opens in new window Total cost No data
