Obiettivo
This proposal aims at developing a versatile fermentation platform for the conversion of lipid feed stocks into diverse added-value products. It is proposed to develop the oleaginous yeast Yarrowia lipolytica into a microbial factory by directing its versatile lipid metabolism towards the production of industrially valuable compounds like wax esters (WE), polyhydroxyalkanoates (PHA’s), free hydroxyl fatty acids (HFA’s) and isoprenoid-derived compounds (carotenoids, polyenic carotenoid ester). Conversion of lipid intermediates into these products will be achieved by introducing heterologous enzyme functions isolated from marine hydrocarbonoclastic bacteria into Yarrowia. To achieve these goals we have assembled a team with a broad set of complementary expertise in microbial physiology, metabolic engineering, yeast lipid metabolism, metagenomics, biochemical and protein engineering. Already available for this project are a number of genetically engineered Yarrowia strains as well as a collection of genes encoding enzymes for the production of WE’s, 3-HFA’s, PHA’s and carotenoids. The following complementary research focus areas are proposed: (1) Engineering of metabolic precursor pools in Yarrowia lipolytica for the production of added-value products from lipids (INRA, UGe). (2) Conversion of metabolic precursor pools in Yarrowia to added-value products by overexpressing heterologous biosynthetic enzymes (UGe, INRA, UoM). (3) Discovery and characterization of novel aliphatic enzyme activities by metagenomic screening of marine hydrocarbonoclastic and other oil- and fat-metabolizing microbial communities (TUBS, UoN). The project is further complemented by: (i) the activity of a professional valorization company (Ascenion) providing IP protection and commercialization services; (ii) by proactive efforts to expand the project’s target products’ application potential (Avecom).
Campo scientifico
- engineering and technologyindustrial biotechnologymetabolic engineering
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- engineering and technologyindustrial biotechnologybiomaterialsbioplasticspolyhydroxyalkanoates
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Parole chiave
Argomento(i)
Invito a presentare proposte
FP7-KBBE-2007-1
Vedi altri progetti per questo bando
Meccanismo di finanziamento
CP-FP - Small or medium-scale focused research projectCoordinatore
9000 Gent
Belgio