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Development of Electrochemical Reactors Using Dehydrogenases for Enantiopure Synthon Preparations

Objective

The aim of the project is the development of electrochemical reactors for the manufacture of fine chemicals with dehydrogenases as a process with almost zero waste emission. The production of enantio pure compounds with high EE’s can be achieved by using dehydrogenases as biocatalysts, because they express high enantio selectivity in ketone reduction, combined with broad substrate spectra by some of these enzymes. These proteins will be engineered for improved catalytic performance using the tools of molecular evolution, modelling, structure prediction, and crystallography. As these dehydrogenases typically require cosubstrate regeneration by aid of a second enzymatic reaction, we are looking for the alternative solution of an electrochemical approach for the regeneration of reduced cofactors. If all active compounds can be functionally immobilized on the electrode surface the constructed reactor would convert the educt in the input flow to the product in the output flow avoiding any contaminations. All necessary components like the mediator, the cofactor and the dehydrogenase will be bound to nano or meso structured electrodes (for increased active surface area) resulting in biofunctionalised surfaces with tailored properties at the nanoscale. Optimization of the electrode materials and surfaces, of the mediators and the required spacers as well as the surface bound dehydrogenase activities will result in electrochemical reactor moduls which can deliver enantio pure synthons for desired compounds in pharmaceutical or agrochemical applications. The obtained data will increase our knowledge on nanostructured catalysts and inorganic-organic hybrid systems. Cheap cofactor regeneration, easy product purification, high selectivity and avoidance of organic solvents will be the advantages of such processes to satisfy the demands of green chemistry in respect of environmentally friendly, flexible and energy efficient productions.

Field of science

  • /natural sciences/earth and related environmental sciences/geology/mineralogy/crystallography
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /natural sciences/biological sciences/molecular biology/molecular evolution
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

FP7-NMP-2007-SMALL-1
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

UNIVERSITAT DES SAARLANDES
Address
Campus
66123 Saarbrucken
Germany
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 890 880
Administrative Contact
Mareike Schmitt (Ms.)

Participants (5)

ECOLE NATIONALE SUPERIEURE DE CHIMIE ET DE PHYSIQUE DE BORDEAUX
France
EU contribution
€ 344 120
Address
16 Avenue Pey Berland
33607 Pessac
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Bernard Berdeu (Prof.)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
EU contribution
€ 385 604
Address
Rue Michel Ange 3
75794 Paris
Activity type
Research Organisations
Administrative Contact
Michel Mauvais (Mr.)
KOBENHAVNS UNIVERSITET
Denmark
EU contribution
€ 593 245
Address
Norregade 10
1165 Kobenhavn
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ivan Kirstoffersen (Mr.)
MIDDLE EAST TECHNICAL UNIVERSITY
Turkey
EU contribution
€ 332 500
Address
Dumlupinar Bulvari 1
06800 Ankara
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ayhan Sitki Demir (Prof.)
IEP GmbH
Germany
EU contribution
€ 203 560
Address
Rheingaustrasse, 190-196
65203 Wiesbaden
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Antje Gupta (Dr.)