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Functional assays for membrane protein on nanostructured supports

Objective

More than 50% of all drug targets are membrane proteins; new research tools to screen function of membrane drug targets are therefore expected to open up new avenues for original drug development. The proposed project addresses the need of the pharmaceutical industry for new technologies for reliable and efficient screening of membrane proteins as drug targets. Most critical current aspects of membrane protein assays are (a) the lack of reliable procedures to immobilize membrane proteins on sensor surfaces in a format suitable for label-free high-throughput screening of drug candidates; (b) the need for downscaling assay formats to accelerate functional screening; and (c) the feasibility of reading out the diverse functions of membrane proteins. The partners – with highly complementary expertise and experience of working together – will develop platforms for functional membrane protein assays by integration of the most recently gained knowledge and techniques. The key concepts of the platforms include (a) exploitation of nanoporous substrates to enhance the stability of supported proteolipid membranes and their integration in a sensor chip format; (b) nanoscale surface modifications for directed self-assembly of proteolipid structures on chip; and (c) self-assembly of proteolipid membranes onto nano-sized sensor structures from proteoliposomes, and demonstration of the functionality in quantitative drug candidate screening assays suitable for commercial applications. The project is expected to make a substantial contribution to (a) improved understanding of lipid membrane and membrane protein interaction with designed nanoenvironments; (b) development of prototype products and intellectual property related to membrane protein sorting and handling; (c) new compounds for functionalization of biosensor applications; (d) cost-effective array-based concepts for nanoplasmonic and electrochemical sensing; and (e) functional assays for membrane protein drug targets.

Field of science

  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /engineering and technology/electrical engineering, electronic engineering, information engineering/electronic engineering/sensors

Call for proposal

FP7-NMP-2007-SMALL-1
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Address
Raemistrasse 101
8092 Zuerich
Switzerland
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 746 920
Administrative Contact
Janos Vörös (Mr.)

Participants (13)

PAUL SCHERRER INSTITUT
Switzerland
EU contribution
€ 312 278
Address
Forschungstrasse 111
5232 Villigen Psi
Activity type
Research Organisations
Administrative Contact
Irene Walthert (Ms.)
CHALMERS TEKNISKA HOEGSKOLA AB
Sweden
EU contribution
€ 515 139
Address
-
41296 Goeteborg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Annika Hofling (Ms.)
Klinik Max Planck Institut für Psychiatrie
Germany
EU contribution
€ 426 167
Address
Hofgartenstrasse 8
80539 Muenchen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Udo Schreiner (Mr.)
IMS bordeaux
France
EU contribution
€ 389 100
Address
351 Cours De La Liberation
33405 Talence
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Didier Lassaque (Mr.)
EOTVOS LORAND TUDOMANYEGYETEM
Hungary
EU contribution
€ 98 160
Address
Egyetem Ter 1-3
1053 Budapest
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Katalin Juhászné Huszty (Dr.)
UNIVERSITEIT TWENTE
Netherlands
EU contribution
€ 382 575
Address
Drienerlolaan 5
7522 NB Enschede
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Gyula Julius Vancso (Prof.)
LEISTER PROCESS TECHNOLOGIES*
Switzerland
EU contribution
€ 278 160
Address
Galileo-strasse 10
6056 Kagiswil
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Marco Di Berardino (Dr.)
Layerlab AB
Sweden
EU contribution
€ 137 099
Address
Stena Center 1D
412 92 Goteborg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Torbjorn Pettersson (Mr.)
SuSoS AG
Switzerland
EU contribution
€ 60 960
Address
Lagerstrasse 14
8600 Dubendorf
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Samuele Tosatti (Dr.)
Farfield Group Limited
United Kingdom
EU contribution
€ 190 320
Address
Farfield House, Southmere Court, Electra Way, Crewe Business Park
CWI 6GU Crewe
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Louise Madden (Ms.)
MIKROVAKUUM MIKROELEKTRONIKAI ES VAKUUMTECHNIKAL KFT
Hungary
EU contribution
€ 157 920
Address
Kerekgyarto U 10
1147 Budapest
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Istvan Szendro (Mr.)
HYDROGENE AB
Sweden
EU contribution
€ 165 000
Address
Martens Falad 102
225 92 Lund
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Per Kjellbom (Prof.)
F. HOFFMANN-LA ROCHE AG
Switzerland
EU contribution
€ 80 300
Address
Grenzacherstrasse 124
4070 Basel
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Michael Hennig (Dr.)