Objectif
More than 50% of all drug targets are membrane proteins; new research tools to screen function of membrane drug targets are therefore expected to open up new avenues for original drug development. The proposed project addresses the need of the pharmaceutical industry for new technologies for reliable and efficient screening of membrane proteins as drug targets. Most critical current aspects of membrane protein assays are (a) the lack of reliable procedures to immobilize membrane proteins on sensor surfaces in a format suitable for label-free high-throughput screening of drug candidates; (b) the need for downscaling assay formats to accelerate functional screening; and (c) the feasibility of reading out the diverse functions of membrane proteins. The partners – with highly complementary expertise and experience of working together – will develop platforms for functional membrane protein assays by integration of the most recently gained knowledge and techniques. The key concepts of the platforms include (a) exploitation of nanoporous substrates to enhance the stability of supported proteolipid membranes and their integration in a sensor chip format; (b) nanoscale surface modifications for directed self-assembly of proteolipid structures on chip; and (c) self-assembly of proteolipid membranes onto nano-sized sensor structures from proteoliposomes, and demonstration of the functionality in quantitative drug candidate screening assays suitable for commercial applications. The project is expected to make a substantial contribution to (a) improved understanding of lipid membrane and membrane protein interaction with designed nanoenvironments; (b) development of prototype products and intellectual property related to membrane protein sorting and handling; (c) new compounds for functionalization of biosensor applications; (d) cost-effective array-based concepts for nanoplasmonic and electrochemical sensing; and (e) functional assays for membrane protein drug targets.
Champ scientifique
- sciences médicales et de la santémédecine fondamentalepharmacologie et pharmaciedécouverte de médicaments
- ingénierie et technologiegénie électrique, génie électronique, génie de l’informationingénierie électroniquecapteursbiocapteurs
- sciences naturellessciences biologiquesbiochimiebiomoléculeprotéines
- sciences naturellessciences biologiquesbiochimiebiomoléculelipides
Programme(s)
Appel à propositions
FP7-NMP-2007-SMALL-1
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Régime de financement
CP-FP - Small or medium-scale focused research project
Coordinateur
8092 Zuerich
Suisse
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Participants (13)
5232 Villigen Psi
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412 96 Goteborg
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80539 Munchen
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33405 Talence
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1053 Budapest
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7522 NB Enschede
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6056 Kagiswil
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412 92 Goteborg
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8600 Dubendorf
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CWI 6GU Crewe
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1147 Budapest
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225 92 Lund
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4070 Basel
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