Generation of stable transgenic embryonic stem cell lines

Información del proyecto

TRANSHESC

Identificador del acuerdo de subvención: 220383

Proyecto cerrado

Fecha de inicio 1 Septiembre 2008

Fecha de finalización 31 Agosto 2010

Financiado con arreglo a

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Coste total € 243 268,29

Aportación de la UE € 243 268,29

Coordinado por

THE UNIVERSITY OF SHEFFIELD
United Kingdom

Objetivo

Human embryonic stem cells (hESC) are pluripotent cells derived from blastocyst stage early embryos. Since the original publication a vast number of studies have described the differentiation of hESC and their potential in regenerative medicine. It is now well known that hESC might be used in cell replacement therapies in a number of severe degenrative diseases like Type I diabetes or Parkinson’s disease. In addition, hESCs and their derivatives could be used as tools in discovering new drugs and in understanding mechanisms of genetic diseases. HESC applications could also be used in toxicological studies reducing the need of animal experiments. Genetic manipulation of hESC will be a crucial issue in a variety of future studies. To elucidate the role of any given gene for example in directed differentiation or pathogenesis the loss- and gain-of gene function approaches are of major importance. Also, considering the potential therapeutic applications of the hESC their genetic modifications may be necessary. The first part of this proposal is designed to improve and test novel efficient and safe transposon-based gene transfer method in generating conditional transgenic hESC lines. Of special interest is the use of insulator elements around transgene to assure nonbiased expression of the integrated sequences. Several reports have indicated that in long term hESC cultures the cells aquire chromosomal aberrations. The observed karyotypic changes probably reflect the progressive adaptation of self-renewing cells to their culture conditions. This process has parallels with malignant transformation. Changes observed in hESC in culture reflect tumorigenic events that occur in vivo, particularly in testicular germ cell tumors (TGCT). A recent report indicated the formation of a chromosomal homogeneous staining region (HSR) in some hESC lines, a feature almost a hallmark of cancer cells. The HSR represents an amplified small chromosomal region most likely containing gene

Ámbito científico

• medical and health sciences › medical biotechnology › genetic engineering › gene therapy
• medical and health sciences › clinical medicine › endocrinology › diabetes
• medical and health sciences › medical biotechnology › cells technologies › stem cells
• medical and health sciences › basic medicine › neurology › parkinson
• medical and health sciences › clinical medicine › embryology

Palabras clave

• adaptation
• human embryonic stem cells
• self-renewal
• transgenic

Programa(s)

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Tema(s)

• PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development"

Convocatoria de propuestas

FP7-PEOPLE-2007-2-1-IEF
Consulte otros proyectos de esta convocatoria

Régimen de financiación

MC-IEF - Intra-European Fellowships (IEF)

Coordinador

THE UNIVERSITY OF SHEFFIELD

Contacto administrativo

Deborah Mcclean (Ms.)

• Aportación de la UE: € 243 268,29
• Dirección: FIRTH COURT WESTERN BANK ; S10 2TN Sheffield ; Reino Unido
• Región: Yorkshire and the Humber › South Yorkshire › Sheffield
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: Sin datos