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Combinatorial synthesis of peptide arrays with a laser printer

Objective

Our particle-based method allows us to synthesise high complexity peptide arrays by combinatorial synthesis and for an unrivalled prize. We plan to further develop this new technology up to the level of robust prototype machines, and mate it to bioinformatics and readout tools. Together, our procedure(s) should boost the field of proteomics in a similar way as the lithographic technologies did with the field of genomics. Central to our novel method are the activated chemical building blocks that are “frozen” within solid amino acid particles. Thereby, we can use a colour laser printer to send them to defined addresses on a 2D support, where the particles are simply melted to induce a spatially defined coupling reaction of now freed amino acid derivatives. By repeated printing and melting cycles this simple trick yields high complexity peptide arrays. Based on existing pre-prototypes, we will develop a user-friendly peptide laser printer that spatially defined addresses our 20 different amino acid toners in high resolution to a support (WP1), and a scanner that especially fast and sensitive reads out the large formats delivered by the peptide laser printer (WP2). The increased production of amino acid toners and array supports are other bottlenecks in the output of peptide arrays that are tackled in WP3. This should allow us to increase the output of individual peptide spots from currently 0,5 Million to >10 Million peptides per month. Finally, to foster a market for high complexity peptide arrays, we will work out paradigmatic application examples in WP4. These aim to directly screen for antibiotic or apoptosis inducing D-peptides, and for the comprehensive readout of the different antibodies that patrol the serum of autoimmune patients. Based on user-friendly prototype machines, on first paradigmatic application examples for high complexity peptide arrays, and shielded by a strong patent, the participating SMEs will commercialise this new technology.

Field of science

  • /natural sciences/physical sciences/optics/laser physics
  • /natural sciences/chemical sciences/organic chemistry/amines

Call for proposal

FP7-HEALTH-2007-B
See other projects for this call

Funding Scheme

CP-TP - Collaborative Project targeted to a special group (such as SMEs)

Coordinator

KARLSRUHER INSTITUT FUER TECHNOLOGIE
Address
Kaiserstrasse 12
76131 Karlsruhe
Germany
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 313 766,69
Administrative Contact
Natascha Wallburg

Participants (11)

UPPER AUSTRIAN RESEARCH GMBH
Austria
EU contribution
€ 298 800
Address
Hafenstrasse 47-51
4020 Linz
Activity type
Research Organisations
Administrative Contact
Alois Sonnleitner (Dr.)
FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Germany
EU contribution
€ 300 000
Address
Hansastrasse 27C
80686 Munchen
Activity type
Research Organisations
Administrative Contact
Walter Krause (Mr.)
BIOINFOBANK INSTITUTE
Poland
EU contribution
€ 180 000
Address
Limanowskiego 24/A 16
60744 Poznan
Activity type
Research Organisations
Administrative Contact
Leszek Rychlewski (Dr.)
TECHNICAL UNIVERSITY OF VARNA
Bulgaria
EU contribution
€ 150 000
Address
Studentska 1
9010 Varna
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Peycho Popov (Dr.)
AIMS Scientific Products GmbH
Germany
EU contribution
€ 100 080
Address
Inhoffenstraße 7
38124 Braunschweig
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Norbert Zander (Dr.)
PEPperPRINT GmbH
Germany
EU contribution
€ 100 000
Address
Schlossweg 9
69168 Wiesloch
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Volker Stadler (Dr.)
KMS Automation GmbH
Germany
EU contribution
€ 550 610
Address
Im Webertal 20
78713 Schramberg-waldmoesssingen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Joachim Schullerer (Mr.)
MICROSYSTEMS LTD
Bulgaria
EU contribution
€ 150 000
Address
Chaika 15 D 21
9010 Varna
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Nikolay Nikolov (Mr.)
AKAtech Produktions- und Handels GmbH
Austria
EU contribution
€ 600 000
Address
Untermühlberg 1
4890 Frankenmarkt
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Michael Farthofer (Mr.)
Selvita spolka z ograniczona odpowiedzialnoscia
Poland
EU contribution
€ 99 960
Address
Ul. Gersona 2
30818 Krakow
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Justyna Supel (Ms.)
DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG

Participation ended

Germany
EU contribution
€ 145 233,31
Address
Im Neuenheimer Feld 280
69120 Heidelberg
Activity type
Research Organisations
Administrative Contact
Bettina Crispin (Ms.)