Final Report Summary - REGULATORYCIRCUITS (Novel Systematic Strategies for Elucidating Cellular Regulatory Circuits)
We use the budding yeast model organism, which allows for efficient manipulations, to dissect transcriptional responses that are prototypical of many regulatory networks in living cells. Our model is stress response to change in external environment which is mediated by multiple signaling pathways and several transcription factors. Many of these components are conserved all the way to human, although the pathways have adapted to respond to different stimuli in humans.
Our approach aims to elucidate mechanisms that are opaque to classical screens and facilitate building detailed predictive models of these responses. These results will lead to understanding of general principles that govern transcriptional networks. This is the first approach to comprehensively characterize the molecular mechanisms that modulate a transcriptional response, and arrange them in a coherent network. It will open many questions for detailed biochemical investigations, as well as set the stage to extend these ideas to use more detailed phenotypic assays and in more complex organisms.
We use the budding yeast model organism, which allows for efficient manipulations, to dissect transcriptional responses that are prototypical of many regulatory networks in living cells. Our model is stress response to change in external environment which is mediated by multiple signaling pathways and several transcription factors. Many of these components are conserved all the way to human, although the pathways have adapted to respond to different stimuli in humans.
Our approach aims to elucidate mechanisms that are opaque to classical screens and facilitate building detailed predictive models of these responses. These results will lead to understanding of general principles that govern transcriptional networks. This is the first approach to comprehensively characterize the molecular mechanisms that modulate a transcriptional response, and arrange them in a coherent network. It will open many questions for detailed biochemical investigations, as well as set the stage to extend these ideas to use more detailed phenotypic assays and in more complex organisms.