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Modulation of CFTR stability and function from the extracellular space

Objective

The CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) is a unique ABC protein functioning as a chloride channel. It consists of two transmembrane and two nucleotide binding domains, and a regulatory region. The cftr gene is mutated in cystic fibrosis, an inherited disease of high morbidity and mortality mostly affecting people with European ancestors. So far more than 1,500 mutations are known affecting the stability or the function of the protein that results in the lack of functional channels in the cell membrane. This leads to imbalanced water and salt homeostasis affecting the function of all organs with secretory epithelia. Therefore it is important to develop therapeutic agents to stabilize CFTR and restore its function. This is being done by blind screening resulting in compounds that are hydrophobic and act on either the transmembrane or intracellular parts of the protein. In this study we aim to modulate the stability and function of CFTR from the extracellular side. The advantage of this approach is the targeting of the hydrophilic extracellular loops that enable structural studies and design of water soluble drugs with advantageous toxological properties. We will employ cutting-edge computational approaches combined with solid experimental methods to gain insights in the structure and dynamics of the larger extracellular loops in CFTR. The effects of the extracellular loops on the conformation of the transmembrane region and the protein function will be studied that will establish a basis for rational drug design. Our preliminary results indicate that the extracellular loops possess stable secondary structural elements and changes in their conformation can either stimulate or inhibit the protein function. Together, the extracellular loops will provide a focused target to restore CFTR stability and function. Moreover, this approach can be also applied to rationally modulate the function of other ABC proteins involved in human diseases.

Field of science

  • /medical and health sciences/basic medicine/physiology/homeostasis
  • /natural sciences/biological sciences/genetics and heredity/mutation
  • /natural sciences/biological sciences/genetics and heredity/nucleotide
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /social sciences/sociology/demography/mortality

Call for proposal

FP7-PEOPLE-IRG-2008
See other projects for this call

Funding Scheme

MC-IRG - International Re-integration Grants (IRG)

Coordinator

Office for Research Groups Attached to Universities and Other Institutions of the Hungarian Academy of Sciences
Address
Teréz Krt. 13
1067 Budapest
Hungary
Activity type
Research Organisations
EU contribution
€ 75 000
Administrative Contact
Ilona Berzéné Pénzes