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Content archived on 2024-05-29

Histone modifications and DNA methylation mapping on the entire Human Chromosome 21

Objective

As the sequencing of the human genome nears completion, the challenge that faces the scientific community is to decipher the underlying meaning behind these precisely ordered nucleotides. To exploit the vast amount of information now available from the hum an genome sequencing effort, new strategies focused on understanding gene regulation are being developed. One excellent model for these studies is the Human Chromosome 21 (HC21). This chromosome is the smallest auto-some and for this reason, and its clinical relevance in Trisomy 21, is probably the most well-studied chromosome. HC21 has been at the forefront of genomics research in the development of genetic and physical maps, sequencing, gene identification and characterization, and gene expression. In the nucleus of all eukaryotic cells, DNA is highly folded, constrained and compacted by histone proteins in a polymer called chromatin, itself rearranged in the final compacted chromosome. This structure is dynamic and participates in many different fundament al processes of the cell. In recent years it has become clear that chromatin function is extensively modulated by epigenetic modifications, which proceed primarily in the amino-terminal tails of histones and in methylation events of DNA sequence. In our p roject we aim to identify Histones and DNA modifications associated with HC21 transcriptional activity, in a large-scale fashion, using the new exciting ChIP-on-chip approach. This technology has begun to provide insights into the genome-wide study of cell s transcriptional programs, by combining the technique of chromatin immunoprecipitation (ChIP) with hybridization to DNA micro-arrays (chip). In this way, we will establish a precise correlation between epigenetic features and gene expression on the whole h uman chromosome 21. Our project apprehends globally HC21 activity and will offer, at scientific community's disposal, a huge structure/function analysis of an entire human chromosome.

Fields of science (EuroSciVoc)

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Keywords

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Topic(s)

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Call for proposal

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FP6-2002-MOBILITY-5
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

EIF - Marie Curie actions-Intra-European Fellowships

Coordinator

FACULTE DE MEDECINE - GENEVA UNIVERSITY
EU contribution
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Address


Switzerland

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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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