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Directed Evolution of Enzymes that Modify Histone Tails

Objectif

The modification of chromatin structure (e.g. by acetylation or methylation of the histone tails) plays a central role in transcriptional regulation, constituting an epigenetic code. Currently the dissection of the roles of individual modified histone residues is limited by low activity and/or specificities of isolated/recombinant histone modifying enzymes. We propose to combine directed evolution with biochemical and in vivo assays to understand the functional consequences of modifying specific histone residues. Natural histone-modifying enzymes will be used as starting points to evolve enzymes with have enhanced activities and altered specificities to probe biological function.

Champ scientifique (EuroSciVoc)

Mots‑clés

Thème(s)

Appel à propositions

FP6-2004-MOBILITY-5
Voir d’autres projets de cet appel

Régime de financement

EIF - Marie Curie actions-Intra-European Fellowships

Coordinateur

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
Contribution de l’UE
Aucune donnée
Coût total
Aucune donnée