Objective
The main objective of the project is to generate antibodies that specifically bind to human tumour vasculature. This will be achieved by ex vivo perfusion of surgically resected livers containing tumours, with phage display libraries. The phage display technology has already been applied successfully in in-vivo selection for tumour vasculature in mice. However, a disadvantage of this system is that while the tumour cells can be human, the vasculature is of mouse origin, and the human tumour vasculature antigens will differ.
Selection of phage displayed antibodies by perfusion of tumour-bearing organs is novel because it will give direct access to living human tumour vasculature. Using resected human liver segments, we have established a perfusion system, and demonstrated that phage are delivered to the tumour vasculature as demonstrated by immunohistochemistry.
To ensure the highest possible success rate in the perfusion-selections, we have characterised different phage display systems for display efficiency and optimised phage production protocols The selected antibodies will initially be investigated in established immunohistochemical assays, and subsequently tested for potential tumour vascular targeting using resected tumour-bearing organs in our perfusion system. The perfusion-selection of phage-display libraries described offers a unique opportunity to generate clinically relevant antibodies or peptides for anti-vascular cancer therapy and to identify important human tumour vascular antigens.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
- natural sciencesbiological sciencesmicrobiologyvirology
- natural sciencesbiological sciencesbiochemistrybiomolecules
- medical and health sciencesclinical medicineoncology
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Call for proposal
FP6-2004-MOBILITY-5
See other projects for this call
Funding Scheme
EIF - Marie Curie actions-Intra-European FellowshipsCoordinator
LONDON
United Kingdom