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Content archived on 2024-05-28

MicroRNA and metabolic programming in rainbow trout

Objective

Carnivorous fish require high-protein intake for protein synthesis and energy purposes and are currently fed high-protein diets based mainly on fishmeal. However, fish meal replacement by carbohydrates is important for sustainable aquaculture in order to decrease nitrogen waste in aquatic environment and preserve marine resources. Unfortunately, rainbow trout is considered as poor user of dietary carbohydrates. Thus, understanding how metabolism is regulated in carnivorous fish constitutes the first objective of our proposal. The second is to determine whether early metabolic programming may help us to tailor fish metabolism to new sustainable diets.

MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate protein expression mainly by targeting the 3’ UTR region of mRNAs. miRNAs have been involved in the regulation of metabolism related gene expression as well as in the control of insulin/amino acids signalling.

The main objective of this project is to analyze the nutritional regulation and metabolic programming of hepatic microRNA levels in rainbow trout. The study will be first focussed on the miRNA122 which is characteristic from the liver and involved in the regulation of cholesterol and triglycerides synthesis. Two important research projects concerning the whole sequencing of the rainbow trout genome and the analysis of the differential expression of the miRNA profile in different tissues of rainbow trout at different nutritional status are currently in progress and will give us the opportunity to enlarge the study to other interesting miRNA for hepatic metabolism and find target genes for these miRNA.
Our proposal will be divided in three tasks:
1. Nutritional and hormonal regulation of hepatic miRNAs levels.
2. Time-course evolution of hepatic miRNAs during fish development and growth.
3. Influence of metabolic programming on hepatic miRNA levels and metabolism control.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

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FP7-PEOPLE-2010-IEF
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Funding Scheme

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MC-IEF - Intra-European Fellowships (IEF)

Coordinator

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
EU contribution
€ 197 064,00
Address
147 RUE DE L'UNIVERSITE
75007 PARIS CEDEX 07
France

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Region
Ile-de-France Ile-de-France Paris
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Research Organisations
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