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Methods for high-throughput (HTP) analysis of protein glycosylation

Objective

Glycosylation is a post-translational modification that enriches protein complexity and function. Dysregulation of glycosylation is associated with a wide range of diseases, including cancer, diabetes, as well as congenital, cardiovascular, immunological and infectious disorders. A number of studies identified potentially important glycan disease biomarkers. With regard to biotechnology, proper glycosylation of biologicals is important, as deviations in glycosylation are known to be associated with adverse drug reactions and reduced therapeutic efficacy. However, glycomics is significantly lagging behind genomics and proteomics, mainly due to the absence of high-throughput analytical methods which can reliably quantify a multitude of glycan structures in complex biological samples.
We are confident that by coordinated efforts of leading European scientists in glycan analysis using HPLC, MS and CGE-LIF technologies this project will make a decisive step forward by developing real high-throughput tools for glycosylation analysis. By teaming up with leading European researchers in the field of genome wide association studies this project will perform validation of all methods on extremely well characterized set of samples resulting from the FP6 EuroSpan project. The addition of the newly generated glycome data to the pre-existing information about these individuals will enable development of methods for the systems biology approach analysis of the glycome which will integrate glycomic, genomic and environmental data about thousands of individuals. The same methods will also be adapted for quality control and monitoring in the production of biopharmaceuticals.
Strong participation of SMEs in the project and close contacts with large industrial partners will ensure that research accomplishments achieved by collaboration between academic and industrial scientists are swiftly transformed into innovative products and services for the benefit of European industry.

Field of science

  • /medical and health sciences/basic medicine/pharmacology and pharmacy/adverse drug reactions
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /natural sciences/biological sciences/biochemistry/biomolecules/carbohydrates

Call for proposal

FP7-HEALTH-2011-two-stage
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

ACADEMISCH ZIEKENHUIS LEIDEN
Address
Albinusdreef 2
2333 ZA Leiden
Netherlands
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 638 960,80
Administrative Contact
Manfred Wuhrer (Dr.)

Participants (8)

THE UNIVERSITY OF EDINBURGH
United Kingdom
EU contribution
€ 468 680
Address
Old College, South Bridge
EH8 9YL Edinburgh
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Gemma Scott-Wood (Ms.)
Klinik Max Planck Institut für Psychiatrie
Germany
EU contribution
€ 328 100
Address
Hofgartenstrasse 8
80539 Muenchen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Erdmann Rapp (Dr.)
NATIONAL INSTITUTE FOR BIOPROCESSING RESEARCH AND TRAINING LIMITED
Ireland
EU contribution
€ 589 120
Address
Fosters Avenue Mount Merrion
4 Blackrock Dublin
Activity type
Research Organisations
Administrative Contact
Pauline Mary Rudd (Prof.)
GENOS DOO ZA VJESTACENJE I ANALIZU
Croatia
EU contribution
€ 988 579,60
Address
Vatrogasna 112
31000 Osijek
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Gordan Lauc (Dr.)
BIA SEPARATIONS DOO
Slovenia
EU contribution
€ 484 400
Address
Mirce 21
5270 Ajdovscina
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Jana Vidič (Dr.)
LUDGER LIMITED
United Kingdom
EU contribution
€ 1 011 232
Address
Culham Science Centre - Building E1
OX14 3EB Abingdon
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Daryl Fernandes (Dr.)
MP BIOMEDICALS SARL
France
EU contribution
€ 115 800
Address
Rue Jean Geiler De Kaysersberg
67400 Illkirch-graffenstaden
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Miodrag Micic (Dr.)
GLYXERA GMBH
Germany
EU contribution
€ 374 100
Address
Brenneckestrasse 20 Zenit
39120 Magdeburg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Thomas Hermsdorfer (Mr.)