Objectif A novel analytical platform is proposed to detect and identify DNA at low concentration in a high throughput manner at the single molecule level.The potential impact of this research is significant and will result in single molecule detection becoming a mainstream tool within the medical diagnostics and analytical communities. 'Rare event' detection plays an important role in the early detection of illnesses and disease (e.g. cancers and bacterial infections). Using analytical technologies that exist today it is almost impossible to detect a single DNA strand within a standard blood sample (of a few mLs) within a reasonable time frame. The technology that will be developed within the current project will allow for such detection to be performed both rapidly and efficiently. If successful, the core technology described will become a mainstream analytical tool that will be of significant benefit within biomedical laboratories, hospitals, and clinics around the world.Specifically, chemical and semiconductor processing methods will be developed to define a novel approach to high throughput DNA quantification at the single molecule level. This innovative technology will function by introducing biological samples in micro- and nanofluidic chips and using electric fields to direct DNA strands through nanometre-sized pores on a membrane. Detection and sizing of the individual DNA strands (labelled with fluorophores) is then accomplished using confocal fluorescence spectroscopy.This new approach to high-throughput, single molecule DNA analysis harnesses the strengths of both analytical spectroscopy and silicon fabrication technology to allow the creation of hybrid devices in which molecular quantification can be realized. I expect this work to have major impact and open up new possibilities for nano-analytical tools in the chemical and biological sciences. Champ scientifique natural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesopticsspectroscopyemission spectroscopymedical and health sciencesclinical medicineoncologynatural sciencesphysical scienceselectromagnetism and electronicssemiconductivitynatural scienceschemical sciencesinorganic chemistrymetalloids Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-SG-PE4 - ERC Starting Grant - Physical and Analytical Chemical sciences Appel à propositions ERC-2011-StG_20101014 Voir d’autres projets de cet appel Régime de financement ERC-SG - ERC Starting Grant Institution d’accueil IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE Contribution de l’UE € 1 497 620,00 Adresse SOUTH KENSINGTON CAMPUS EXHIBITION ROAD SW7 2AZ LONDON Royaume-Uni Voir sur la carte Région London Inner London — West Westminster Type d’activité Higher or Secondary Education Establishments Contact administratif Brooke Alasya (Ms.) Chercheur principal Joshua Edel (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE Royaume-Uni Contribution de l’UE € 1 497 620,00 Adresse SOUTH KENSINGTON CAMPUS EXHIBITION ROAD SW7 2AZ LONDON Voir sur la carte Région London Inner London — West Westminster Type d’activité Higher or Secondary Education Establishments Contact administratif Brooke Alasya (Ms.) Chercheur principal Joshua Edel (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée