Objective Artificial insemination (AI) in livestock has made it possible to optimize reproduction efficiency. AI is undoubtedly the management technique that has most contributed to the genetic improvement of livestock in modern animal production. By comparison to the other currently available preservation methods of semen, cryopreservation is an established industry used worldwide for performing AI, as it can preserve cells life undefinitely. The main disadvantage of semen cryopreservation is that freezing and thawing induce several forms of cellular lesions, which reduce dramatically the quality and subsequent fertilizing ability of the semen. Sperm motility, viability, membrane integrity and biochemical parameters are all routinely used tests to assess the quality of frozen-thawed semen. However, acceptable motility and viability of spermatozoa do not necessarily lead to acceptable conception rates. By this research project, we aim at increasing the conception rate in ewes following AI, by using frozen-thawed semen. In order to achieve this goal, we have the following objectives: to improve the freezing technologies, to evaluate the cytological and cryobiological indexes of the spermatozoa, to obtain accurate biochemical and electronomicroscopical profiles of the cryopreserved cells, and to test in vivo the efficiency of different preserving and AI technologies, on the cryobiological parameters and reproduction indexes. Fields of science medical and health sciencesclinical medicinegynaecologyreproductive medicinesocial sciencessociologydemographyfertilityagricultural sciencesanimal and dairy sciencedomestic animalsanimal husbandry Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2011-IRSES - Marie Curie Action "International Research Staff Exchange Scheme" Call for proposal FP7-PEOPLE-2011-IRSES See other projects for this call Funding Scheme MC-IRSES - International research staff exchange scheme (IRSES) Coordinator UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA DIN BUCURESTI Address Bd marasti 59 sector 1 011464 Bucuresti Romania See on map Region Macroregiunea Trei Bucureşti-Ilfov Bucureşti Activity type Higher or Secondary Education Establishments Administrative Contact Laura Daniela Urdes (Dr.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution No data Participants (3) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITATEA DE MEDICINA SI FARMACIE CAROL DAVILA DIN BUCURESTI Romania EU contribution € 29 400,00 Address Dionisie lupu 37 020021 Bucuresti See on map Region Macroregiunea Trei Bucureşti-Ilfov Bucureşti Activity type Higher or Secondary Education Establishments Administrative Contact Tudor Savopol (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Other funding No data UNIVERSITATEA OVIDIUS DIN CONSTANTA Romania EU contribution € 114 500,00 Address Mamaia avenue 124 900527 Constanta See on map Region Macroregiunea Doi Sud-Est Constanţa Activity type Higher or Secondary Education Establishments Administrative Contact Stela Filofteia Zamfirescu (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Other funding No data UNIVERSITA DEGLI STUDI DEL MOLISE Italy EU contribution € 29 400,00 Address Via de sanctis ioc. vazzieri 86100 Campobasso See on map Region Sud Molise Campobasso Activity type Higher or Secondary Education Establishments Administrative Contact Salvatore Passarella (Prof.) Links Contact the organisation Opens in new window Other funding No data