Objective Antimicrobial resistance is a global public health problem. Bacteria can quickly develop resistance against many of the existing antimicrobials, and consequently prolonged treatment periods lead to human suffering, loss of productivity, and high health care costs. In order to tackle the antibiotic resistance problem, either new antibiotics or methods for using the current collection of antibiotics more efficiently is necessary. Here, I propose an experimental study to uncover the evolution of bacterial drug resistance which I believe will be very valuable for slowing down the spread of resistance by using the currently available antibiotics. The specific aims of this proposal are: (1) Measuring the rates of evolution under single drug and drug combination treatments; (2) Examining pleiotropic effects of (non-drug) stressful conditions on evolution of drug resistance; (3) Measuring the epistatic interactions between mutations that confer antibiotic resistance. We will perform these studies by using high-throughput measurements and a custom made fully automated fluidics system, “the morbidostat”, which can unequivocally follow bacterial evolution in antibiotic environments. Being able to understand the evolution of antibiotic resistance both at the population and enzymatic level, and identifying the pleitropic effects that cause antibiotic resistance will be very helpful for planning interventions to combat antibiotic resistance. Fields of science medical and health scienceshealth sciencespublic healthnatural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticsmutationmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantibioticsmedical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistance Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2011-CIG - Marie-Curie Action: "Career Integration Grants" Call for proposal FP7-PEOPLE-2011-CIG See other projects for this call Funding Scheme MC-CIG - Support for training and career development of researcher (CIG) Coordinator SABANCI UNIVERSITESI Address Orta mahalle universite caddesi n 27 tuzla 34956 Istanbul Türkiye See on map Region İstanbul İstanbul İstanbul Activity type Higher or Secondary Education Establishments Administrative Contact Aslihan Eran (Ms.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution No data
