Objective
The nuclear pore complex (NPC) is one of the most intricate components of eukaryotic cells and is assembled from ~30 Nucleoporins (Nups) in an unknown manner. Here I present an experimental result driven hypothesis, namely that the composition of the NPC varies across human cell types. As a consequence, the nucleocytoplasmic transport system might be fine-tuned to fulfill specific tasks in various cell types and to adjust the composition of the nuclear compartment. In the research proposed here, cell-type specific structural changes of NPCs will be monitored using systems approaches. The strength of single molecule methods such as cryo electron tomography (cryoET) will be synergistically combined with the strength of mass spectrometry (MS) to measure protein dynamics across cellular states. The outcome will be an atlas of the human NPC containing cell-type and functional state specific structural properties, namely nucleoporin copies per NPC, spatial restraints of protein interfaces, subunit positioning, and shape information. This research will facilitate the generation of a common structural modeling framework by providing the critical information of composition and spatial arrangement. It will furthermore elucidate how NPC composition and structure is adjusted as a function of the biological state of the cell. The long term goal is to integrate the different types of structural data using fitting, docking, and topological modeling approaches in order to project functionally specific compositional data onto structural models of the human NPC.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences chemical sciences analytical chemistry mass spectrometry
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
ERC-2012-StG_20111109
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Host institution
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.