Objective There are several bottlenecks that hinder certain aspects of proteomics, in particular, incompatibility of high throughput technologies with certain protein types or modifications, low sensitivity and lack of quantitative data. I have developed a microfluidics affinity assay compatible with transmembrane proteins and post-translational modifications that is highly sensitive and can provide quantitative data.The primary objective of this proposal is to bioengineer, using the abovementioned building blocks, a multi-functional microfluidic-based human protein arrays. The platform will enable addressing important scientific questions not otherwise possible. Specifically, the process of DNA demethylation, which is poorly characterised due to technological limitations. The biological aspects of chromatin methylation and their regulators that are crucial for cell differentiation and disease will be studied.Work in MuDLOC will include the following: i) Bioengineering of a microfluidic-based platform that expresses thousands of human genes; ii) Design new tools for post-translational modifications and chromatin modifications; iii) Search for chromatin modifiers and their regulators; and iv) Exploration of specific inhibitors using a microfluidic inhibitor screen.Beyond studying chromatin methylation from a new perspective, MuDLOC will greatly benefit a plethora of disciplines, such as proteomics, genomics and cancer research. At the end of the project my vision is to capture under one platform a whole pathway, including protein interactions, post-translational modifications and chromatin modifications. Fields of science natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesgeneticsDNAengineering and technologyother engineering and technologiesmicrotechnologylab on a chipmedical and health sciencesclinical medicineoncology Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) ERC-SG-LS9 - ERC Starting Grant - Applied life sciences and biotechnology Call for proposal ERC-2012-StG_20111109 See other projects for this call Funding Scheme ERC-SG - ERC Starting Grant Host institution BAR ILAN UNIVERSITY EU contribution € 1 497 990,00 Address BAR ILAN UNIVERSITY CAMPUS 52900 Ramat Gan Israel See on map Activity type Higher or Secondary Education Establishments Principal investigator Doron Gerber (Dr.) Administrative Contact Estelle Waise (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Beneficiaries (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all BAR ILAN UNIVERSITY Israel EU contribution € 1 497 990,00 Address BAR ILAN UNIVERSITY CAMPUS 52900 Ramat Gan See on map Activity type Higher or Secondary Education Establishments Principal investigator Doron Gerber (Dr.) Administrative Contact Estelle Waise (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
