Objective
Pseudomonas aeruginosa is a human pathogen causing life-threatening nosocomial infections. Its virulence mechanisms are pleiotropic, but secretion systems have a key role in transporting effectors, toxins and other virulence factors from the bacteria into the environment or target host cells. In recent years, a novel secretion system, the Type VI secretion system (T6SS), was shown to be important for P. aeruginosa virulence. Of three T6SSs encoded on the P. aeruginosa genome, the H1-T6SS is important for P. aeruginosa to compete with other bacteria and to establish chronic infections. H1-T6SS is involved in the secretion of at least three bacteriolytic effectors, Tse1-3, whereas no effectors have been identified for H2- and H3-T6SS. We hypothesise that proteins secreted by these two systems mediate other important aspects of P. aeruginosa virulence and their identification and characterisation are crucial to further understanding the H2- and H3-T6SS function and P. aeruginosa pathogenesis. This project will identify these effectors, using three approaches: 1) identification of regulatory elements controlling H2- and H3-T6SS gene expression in order to up-regulate expression of the H2- or H3-T6SS clusters and other co-regulated genes; 2) analysis of H1-, H2- and H3-T6SS gene expression in biofilm conditions using flowcells; 3) identification of H2- and H3-T6SS secreted effector proteins via secretome analysis of strains that have up-regulated expression of H2 and H3-T6SS clusters. The discovery of these secreted factors will be a breakthrough, since only a very limited number of T6SS substrates have been identified. It will give new insights into P. aeruginosa virulence and could be exploited for development of new therapeutic options.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-IIF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
SW7 2AZ London
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.