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Content archived on 2024-06-18

Identification of Sclerotinia sclerotiorum Effector Proteins mediating virulence on Arabidopsis thaliana ecotypes

Objective

Sclerotinia sclerotiorum is a necrotrophic fungus notorious for its broad host range, including notably oilseed rape, sunflower, lettuce, carrot, onion and potato. In most cases there is no variety with significant resistance or tolerance known to date. Small secreted proteins and low molecular weight metabolites have been reported in several necrotrophic fungi as critical for virulence. However, the role of these effector proteins in S. sclerotiorum virulence is largely unknown, and the molecular mechanisms by which S. sclerotiorum promotes host susceptibility remain poorly understood. We recently discovered that susceptibility to S. sclerotiorum is highly variable among A. thaliana accessions and our preliminary analyses on S. sclerotiorum genome sequence identified promising effector candidates. The objective of this proposal is to validate the function of S. sclerotiorum effector candidates in altering plant cells to promote susceptibility. We will (i) document the diversity of S. sclerotiorum effector protein candidates; (ii) identify effector candidates that promote susceptibility to S. sclerotiorum and (iii) identify effector candidates required for S. sclerotiorum virulence. This project will have a significant impact on our understanding of plant-microbe interactions by providing insights into key molecular processes regulating susceptibility to a necrotrophic pathogen. Besides, it will open new perspectives toward the development of tolerant crop varieties.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

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FP7-PEOPLE-2012-CIG
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-CIG - Support for training and career development of researcher (CIG)

Coordinator

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
EU contribution
€ 100 000,00
Address
147 RUE DE L'UNIVERSITE
75007 PARIS CEDEX 07
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
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Total cost

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