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Identification of Sclerotinia sclerotiorum Effector Proteins mediating virulence on Arabidopsis thaliana ecotypes

Objetivo

Sclerotinia sclerotiorum is a necrotrophic fungus notorious for its broad host range, including notably oilseed rape, sunflower, lettuce, carrot, onion and potato. In most cases there is no variety with significant resistance or tolerance known to date. Small secreted proteins and low molecular weight metabolites have been reported in several necrotrophic fungi as critical for virulence. However, the role of these effector proteins in S. sclerotiorum virulence is largely unknown, and the molecular mechanisms by which S. sclerotiorum promotes host susceptibility remain poorly understood. We recently discovered that susceptibility to S. sclerotiorum is highly variable among A. thaliana accessions and our preliminary analyses on S. sclerotiorum genome sequence identified promising effector candidates. The objective of this proposal is to validate the function of S. sclerotiorum effector candidates in altering plant cells to promote susceptibility. We will (i) document the diversity of S. sclerotiorum effector protein candidates; (ii) identify effector candidates that promote susceptibility to S. sclerotiorum and (iii) identify effector candidates required for S. sclerotiorum virulence. This project will have a significant impact on our understanding of plant-microbe interactions by providing insights into key molecular processes regulating susceptibility to a necrotrophic pathogen. Besides, it will open new perspectives toward the development of tolerant crop varieties.

Ámbito científico (EuroSciVoc)

Programa(s)

Tema(s)

Convocatoria de propuestas

FP7-PEOPLE-2012-CIG
Consulte otros proyectos de esta convocatoria

Régimen de financiación

MC-CIG - Support for training and career development of researcher (CIG)

Coordinador

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
Aportación de la UE
€ 100 000,00
Coste total
Sin datos