Objective
We will develop a new platform and assay for the detection of HIV p24 antigen in serum or blood. The advantages of a p24 test are that it can detect HIV in an early stage of the infection, before antibodies develop, and that it is quantitative. The DETECTHIV project aims at developing an extremely simple viral load test with only one reactant (grafted colloids). In a first phase, a magnetic nanoparticles assay will be developed for use in a microtiter plate with as goal a detection limit of 1 ng/ml. In a second phase, the use of nanoparticles on a microfluidic chip will permit to detect p24 to levels as low as 0.1 picog/ml, one to two orders of magnitude more sensitive than classical Enzyme Linked Immuno-Sorbent Assay (ELISA) p24 tests. Validation of our platform will be both done with recombinant p24 samples and patient samples.
The principle of our test consists in optically detecting the formation of a colloidal gel of magnetic nanoparticles ('agglutination test'). The gel forms in a magnetic field under the presence of antigens that are capable of linking irreversibly two colloidal particles. Therefore the latter are grafted with antibodies that are specific to the p24 antigen. An agglutination test requires only one step with only one reactant and the detection is achieved through simple optical absorbance measurements, owing to the strong optical scattering modification when passing from nanometric colloids to the gelled state.
In the microfluidic chip test, a sample solution of serum or blood is transported through a suspension of magnetic nanoparticles that are magnetically suspended within a microfluidic channel. When brought into a magnetic field, the particles will be able to approach each other, form chains and will be irreversibly linked if the p24 antigen is present. Subsequently, on-chip light scattering techniques will be used to quantify the concentration of permanent chains or clustered beads, which is proportional to the antigen concentration'.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences condensed matter physics soft matter physics
- medical and health sciences health sciences infectious diseases RNA viruses HIV
- engineering and technology nanotechnology nano-materials
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-LIFESCIHEALTH-6
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LAUSANNE
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.