Objective
Aging is a biological process that is not yet understood. The free radical hypothesis of aging states that is a causal relationship between the oxidation status and lifespan such that the progressive deterioration of aging organisms is a consequence of cumulative oxidation damage. In line with this, intracellular accumulation of misfolded and oxidatively damaged proteins, e.g. carbonylated proteins, has repeatedly been associated with aging and the diseases of aging. Such accumulation can be counteracted by over-expression of molecular chaperones and proteases, e.g. the Heat shock proteins (HSPs), and also of oxidant defence proteins like Superoxide dismutase (SOD).
Interestingly, elevated levels of specific HSPs as well as SOD can also prolong lifespan markedly. For example, the Tanguay lab has demonstrated that over-expression of HSP22 in mitochondria of motorneurons of the fruitfly Drosophila melanogaster increases the mean lifespan by over 30% and resistance to oxidative stress by 35%, and that the ef fect was cell type specific. In addition to carbonylation, the formation of advanced glycation end-products (AGEs) on proteins has been demonstrated to increase with age and age-related diseases and to destroy protein function and structure. AGEs are mostly formed via oxidative reactions such as carbohydrate and lipid oxidation and can therefore serve as a biomarker of those events.
In this project I will use Drosophila to address the following questions:
1) Do the levels and localization patterns of protein carbonylation and HSPs in cells and tissues differ between; a) young and old flies, b) short lived versus long lived flies.
2) Is overproduction of HSP22 and other HSPs (e.g. HSP70) reducing protein carbonylation and if so, in what tissues?
3) Is AGE-formation tissue and celltype specific in Drosophila?
I will use the bioimaging and immunocytochemistry techniques available at the Tanguay lab together with the proteomic tools I have applied during my PhD work.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences electrochemistry electrolysis
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences developmental biology
- natural sciences biological sciences biochemistry biomolecules carbohydrates
- medical and health sciences clinical medicine embryology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-6
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
OIF - Marie Curie actions-Outgoing International Fellowships
Coordinator
100 GÖTEBORG
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.