Objective
One of the main control points of programmed cell death affects the release of cytochrome c and other proapoptotic factors from the mitochondria. The detailed mechanism by which cytochrome c is completely and rapidly liberated to the cytosol is not yet well understood, but it appears to involve two steps: i) redistribution of the cytochrome c stored in the mitochondrial cristae and ii) selective permeabilization of the outer mitochondrial membrane. tBid, a proapoptotic protein of the Bcl-2 family, has been recently related to the lipidic reorganization that takes place at the mitochondria during apoptosis. Addition of tBid to mitochondria induces quick morphological changes, which interconnect cristae and make cytochromec available at the outer mitochondria l membrane.
This activity seems to depend on interactions with the mitochondrial lipid cardiolipin, which, being an anchor to cytochrome c at the inner mitochondrial membrane, redistributes during apoptosis, and mediates the selective targeting of tBid to the outer mitochondrial membrane. Recently, it has been observed by epifluorescence microscopy that tBid induces the appearance of novel cardiolipin micro-domains in monolayers. Single molecule optical techniques have been applied to study raft-associated proteins and lipid mobility in vivo and in vitro.
Fluorescence correlation spectroscopy uses the temporal dimension and constitutes a powerful tool to study lipid and protein dynamics in domain-forming membranes. It is the objective of this project to characterize the formation of cardiolipin enriched domains induced by the presence of tBid in connection with the process of mitochondrial cytochrome c redistribution that occurs during apoptosis, by means of fluorescence correlation spectroscopy and microscopy in membrane model systems and mitochondria.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences physical sciences optics microscopy confocal microscopy
- natural sciences physical sciences optics spectroscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
DRESDEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.