Objectif The histone variant macroH2A1 (mH2A1) is associated with gene repression but the molecular mechanisms by which it exerts its function are largely unknown. mH2A is recruited to the IL-8 promoter by ATF-2 where it functions as a transcriptional repressor. Interestingly, macroH2A1 has been shown to bind O-acetyl-ADP-ribose (OAADPR).The functional consequences of this interaction are unknown. This proposal will take two distinct approaches to elucidate the function of mH2A1 in the cell. First, this study will address the detailed mechanism by which mH2A regulates the IL-8 promoter. Specifically, the role OAADPR plays in regulating IL8 transcription will be investigated.In a second approach, this study will investigate the function of mH2A1 on a genomic level. In a series of micorarray experiments, the functional role of mH2A1 across the genome will be elucidated. Special attention will be given to the role of OAADPR binding and mH2A1 function in this genome-wide analysis. Champ scientifique natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicineimmunologymedical and health sciencesclinical medicineoncologynatural sciencesbiological sciencesgeneticsgenomes Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.3 - Marie Curie Incoming International Fellowships (IIF) Appel à propositions FP6-2005-MOBILITY-7 Voir d’autres projets de cet appel Régime de financement IIF - Marie Curie actions-Incoming International Fellowships Coordinateur FOUNDATION FOR BIOMEDICAL RESEARCH OF THE ACADEMY OF ATHENS Contribution de l’UE Aucune donnée Adresse 4 Soranou Ephessiou Street ATHENS Grèce Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée
