Objectif In clinical chemistry, pharmaceutical research and biochemistry the precise and accurate quantification of proteins is critically important. The invention of soft-ionization sources such as electrospray and MALDI, has introduced a revolution in protein characterisation owing to the rapid access to protein sequences. However, these techniques are far from yielding precise (1-2%) quantitative information on the proteins. The vision of the project is to achieve a quantum leap in quantitative proteomics by improving its sensitivity and precision by at least one order of magnitude. To achieve this, a completely new concept of quantitative proteomics is proposed: heteroatom tags, ICP MS and isotope dilution analysis. The goal of the project is the specific labelling of proteins with heteroatom tags for accurate and precise protein quantification, and the development of the related analytical technology. These tags should selectively react with particular amino acids or post-translational modification sites of proteins. The most novel idea is a multi-tagging approach. Contrary to the current procedures, a mixture of tags specific for individual functional groups can be used and then all the information can be obtained in one analytical run. Precise protein quantification may become a diagnostic tool and applications in biomedical research may emerge. Profiling of e.g. phosphoproteins in body fluids or tissue homogenates may become of significance in differential diagnosis of cancer or other diseases and may be developed into a tool for individual therapy control. Consequently, a success of the project will have enormous impact on biochemical, clinical and pharmaceutical research. Champ scientifique sciences naturellessciences biologiquesbiochimiebiomoléculeprotéinesprotéomiquesciences socialesscience politiquetransition politiquerévolutionsciences naturellessciences chimiqueschimie organiqueaminessciences naturellessciences chimiqueschimie analytiquespectrométrie de massesciences naturellessciences physiquesoptiquephysique des lasers Mots‑clés Quantitative proteomics elemental mass spectrometry (ICP MS) elemental tagging heteroatom heteroatom/elemental tagging isotope dilution analysis mass spectrometry (ESI and MALDI) Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Appel à propositions FP6-2005-MOBILITY-5 Voir d’autres projets de cet appel Régime de financement EIF - Marie Curie actions-Intra-European Fellowships Coordinateur CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Adresse Rue michel-ange, 3 Paris France Voir sur la carte Liens Site web Opens in new window Contribution de l’UE Aucune donnée
