Objective
The lipid-derived stress signalling molecules known as the assonates (Jams) play important roles in plantdefense against microbial pathogens and herbivores, and in male fertility.
Arabidopsis mutants defective in JAbiosynthesis or signal transudation are deficient in certain defence responses and are male sterile. Although anew mutants involved in JA signalling have been isolated, it is poorly understood how Jams activate plant defensemechanisms.
Studies on torpedoed insole alkaloid (TIA) metabolism in Catharanthus roses have shown that Activates TIA biosynthesis genes via a assonate and elicitor-responsive element (JERE) in their promoters. This JERE interacts with two JA-responsive members of the AP2-domain transcription factor class called Orcas, for Octadecanoid-Responsive Catharanthus AP2-domain proteins. The Arabidopsis genome encodes144 different AP2-domain transcription factors, but none of them is a clear orthologue of the Orcas. TheCatharanthus JERE is active in Arabidopsis and directs gene expression in response to JA, wounding and infection with certain pathogens, indicating that it interacts with JA and pathogen-responsive members of theArabidopsis AP2-domain family. This proposal aims at the isolation of Arabidopsis proteins, which activate gene expression via the JERE, and form therefore activating components of the JA signal transudation pathway. Such putative activating proteins can include transcription factors, as well as proteins, which directly or indirectly activate transcription factors, such as protein kinesis or other signal transudation proteins, or ultimately even the JA receptor. The proposed method is T-DNA activation tagging of a cell line containing a selectable marker controlled by the JERE. This novel adaptation of the T-DNA activation tagging method allows rapid high-throughput screening of Arabidopsiscells for JA signalling components, which can activate gene expression via the JERE.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine andrology
- natural sciences biological sciences biochemistry biomolecules proteins
- social sciences sociology demography fertility
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LEIDEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.