Objetivo The lipid-derived stress signalling molecules known as the assonates (Jams) play important roles in plantdefense against microbial pathogens and herbivores, and in male fertility. Arabidopsis mutants defective in JAbiosynthesis or signal transudation are deficient in certain defence responses and are male sterile. Although anew mutants involved in JA signalling have been isolated, it is poorly understood how Jams activate plant defensemechanisms. Studies on torpedoed insole alkaloid (TIA) metabolism in Catharanthus roses have shown that Activates TIA biosynthesis genes via a assonate and elicitor-responsive element (JERE) in their promoters. This JERE interacts with two JA-responsive members of the AP2-domain transcription factor class called Orcas, for Octadecanoid-Responsive Catharanthus AP2-domain proteins. The Arabidopsis genome encodes144 different AP2-domain transcription factors, but none of them is a clear orthologue of the Orcas. TheCatharanthus JERE is active in Arabidopsis and directs gene expression in response to JA, wounding and infection with certain pathogens, indicating that it interacts with JA and pathogen-responsive members of theArabidopsis AP2-domain family. This proposal aims at the isolation of Arabidopsis proteins, which activate gene expression via the JERE, and form therefore activating components of the JA signal transudation pathway. Such putative activating proteins can include transcription factors, as well as proteins, which directly or indirectly activate transcription factors, such as protein kinesis or other signal transudation proteins, or ultimately even the JA receptor. The proposed method is T-DNA activation tagging of a cell line containing a selectable marker controlled by the JERE. This novel adaptation of the T-DNA activation tagging method allows rapid high-throughput screening of Arabidopsiscells for JA signalling components, which can activate gene expression via the JERE. Ámbito científico medical and health sciencesclinical medicineandrologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinssocial sciencessociologydemographyfertilitynatural sciencesbiological sciencesgeneticsgenomes Palabras clave Arabidopsis DNA activation tagging T T-DNA activation tagging disease resistance jasmonic acid signal transduction Programa(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Tema(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Convocatoria de propuestas FP6-2002-MOBILITY-5 Consulte otros proyectos de esta convocatoria Régimen de financiación EIF - Marie Curie actions-Intra-European Fellowships Coordinador LEIDEN UNIVERSITY Aportación de la UE Sin datos Dirección Rapenburg 70 LEIDEN Países Bajos Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos
