Objective 1. Project summary A consortium of three companies and two academic research units from Czech Republic, Denmark, Germany and The Netherlands are working together to develop commonly a new approach for improving essential steps in the microarray based gene analytical method. Presently this method consists mainly of five parts, namely1. Extraction of the nucleic acids out of biological samples to be analysed,2. Preparation of the relevant target sequences by amplification, cDNA-synthesis and/or labelling,3. Hybridisation of the generated targets on microarray,4. Measurement of the hybridisation signals and5. Analysis of the data. Since hybridisation, signal measurement and data analysis can be combined in an advanced microarray-based gene sensor system, innovative solutions are needed for getting out of the system quantitative results reflecting the real level of the different nucleic acids to be measured in the biological samples. For analysing those samples containing a large number of different sequences a reduction of the complexity of the nucleic acids mixture is needed. This task will be solved by the new nano-biotechnical component to be developed. It will recognise only a certain set of sequences during the extraction procedure and will allow to transfer these nucleic acids without further preparation steps to the microarray chips improving thereby the detection limit of the whole system. As part of an advanced online hybridisation microarray system ("gene sensor") the structured-structured surfaces of these components will also make possible to improve the robustness and reliability of microarray based DNA-analytical investigations, especially if low abundant species of nucleic acid molecules have to be detected and to be quantified in extracts containing an excess of other nucleic acids. This is often the case in biomedical diagnostics and quality control of food and feed products, for measuring gene expression levels of low abundant mRNA species as well as in many other cases of research and bio-analytical routine measurements in clinical and industry. The final goal is to create an integrated system designed to allow rapid, label-free and low cost analyses of DNA or RNA in highly complex samples. Fields of science natural sciencescomputer and information sciencesdata sciencenatural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAsocial sciencespolitical sciencespolitical transitionsrevolutionsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors Keywords Advanced microarray technology Magnetic nano Nanotechnology Selective DNA extraction beads Programme(s) FP6-NMP - Nanotechnologies and nanosciences, knowledge-based multifunctional materials and new production processes and devices: thematic priority 3 under the 'Focusing and integrating community research' of the 'Integrating and strengthening the European Research Area' specific programme 2002-2006. Topic(s) NMP-2002-3.4.4.3 - New generation of sensors, actuators and systems for health, safety and security of people and environment Call for proposal FP6-2002-NMP-1 See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator UNIVERSITAET BREMEN EU contribution No data Address Bibliothekstrasse 1 BREMEN Germany See on map Total cost No data Participants (4) Sort alphabetically Sort by EU Contribution Expand all Collapse all EXIQON A/S Denmark EU contribution No data Address Bygstubben 9 VEDBAEK See on map Total cost No data GENERI BIOTECH SRO Czechia EU contribution No data Address Machkova 587 HRADEC KRALOVE See on map Total cost No data MILTENYI BIOTEC GMBH Germany EU contribution No data Address Friedrich-Ebert-Strasse 68 BERGISCH GLADBACH See on map Total cost No data PLANT RESEARCH INTERNATIONAL B.V. Netherlands EU contribution No data Address Droevendaalsesteeg 1 WAGENINGEN See on map Total cost No data
