Objectif Vast amounts of money are being spent on drugs acting on membrane proteins without knowledge of the molecular details of their mode of action. Even though the genes of membrane proteins are now accessible, solving their structure remains time-consuming an d often unsuccessful. Given their physiological and biomédical importance, this constitutes a major problem in basic life sciences as well as public health care. Tools have been developed to overexpress, solubilize, stabilize, purify and crystallize membra ne proteins, and they are currently being used in large-scale initiatives for structure determination. Unfortunately, most membrane proteins resist one or more of these steps. The objective of IMPS is to develop imaginative, broad-range tools for membrane structural proteomics. The project brings together experts in chemistry, biochemistry, molecular genetics and crystallography and aims at developing original, innovative tools to attack each of these stumbling blocks. A strong core of crystallographers wit h demonstrated expertise in membrane protein structure determination will apply these novel techniques to a representative test set of membrane proteins. The approaches to be developed include original overexpression systems (insect photoreceptor cells and the chloroplast), unconventional surfactants (amphipathic polymers, fluorinated surfactants, novel detergents and additives) and unconventional crystallization systems (lipid cubic phase crystallization, other non-detergent environments, molecular scaffol ding, bicelles). These novel methodologies will be made available to the community through a distributed technological platform including workshops, hands-on training, and dissemination of the new molecules, whose large-scale synthesis and distribution wil l be carried out by a participant SME. The circulation of scientists between IMPS laboratories will guarantee the rapid spread of the new know-how. Champ scientifique natural sciencesbiological sciencesgeneticsnatural sciencesbiological sciencesmolecular biologymolecular geneticsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesearth and related environmental sciencesgeologymineralogycrystallographynatural sciencesbiological sciencesbiochemistrybiomoleculeslipids Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) LSH-2003-1.1.0-1 - Topics for Specific Targeted Research Project in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Appel à propositions FP6-2003-LIFESCIHEALTH-I Voir d’autres projets de cet appel Régime de financement STIP - Specific Targeted Innovation Project Coordinateur CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Contribution de l’UE Aucune donnée Adresse 3, rue Michel-Ange PARIS France Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (6) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire MEDICAL RESEARCH COUNCIL Royaume-Uni Contribution de l’UE Aucune donnée Adresse 20 Park Crescent LONDON Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée RUPRECHT-KARLS-UNIVERSITÄT HEIDELBERG Allemagne Contribution de l’UE Aucune donnée Adresse Seminarstr. 2 HEIDELBERG Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée TARGETING SYSTEM PHARMA France Contribution de l’UE Aucune donnée Adresse 830, chemin de Vergon EGUILLES Voir sur la carte Coût total Aucune donnée UNIVERSITAET DES SAARLANDES Allemagne Contribution de l’UE Aucune donnée Adresse To be completed. SAARBRUECKEN Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITAT KONSTANZ Allemagne Contribution de l’UE Aucune donnée Adresse Universitätsstr. 10 KONSTANZ Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITÉ D'AVIGNON ET DES PAYS DE VAUCLUSE France Contribution de l’UE Aucune donnée Adresse 74, rue Louis Pasteur AVIGNON Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée