Objective
Heparin-like glycosaminoglycans (HLGAGs) play a key role in regulating the biological activity ofseveral proteins in the coagulation cascade along with many other processes of biomedical importance includinggrowth factor interactions, virus entry, and angiogenesis. However, the relationship between structure and activity of HLGAGs is still very poorly understood due to the complexity and heterogeneity of these polymers. A better understanding of heparin-protein interactions will create an opportunity for the discovery of novel therapeutic interventions for a variety of disease states. Carbohydrate microarrays are promising tools to aid in the discovery of oligosaccharides moieties necessary for carbohydrate-binding protein recognition. Here, we propose the novel preparation of microarrays of synthetic heparin oligosacharides (from solution and solid phase synthesis). First, the linker chemistry will be developed in order to attach the heparin molecules to a chip surface.This strategy will be compatible with the protecting-group manipulations required for the synthesis of HLGAGs. Several approaches will be examined to immobilize the synthetic molecules depending on the type of linker used. After developing the adequate technique for attachment, arrays equipped with a set of covalently immobilized heparin oligosaccharides will be prepared. To demonstrate the utility of such arrays, protein-carbohydrate interaction experiments will be carried out using heparin-binding proteins such as Fibroblast Growth Factors (FGF's). Microarrays will be analysed by using array readers and surface plasmon resonance.The development of automated solid phase methods for the synthesis of HLGAGs will be performed in order to enable the rapid generation of oligosaccharides in order to increase the complexity and diversity of heparin microarrays.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences condensed matter physics quasiparticles
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences polymer sciences
- natural sciences biological sciences biochemistry biomolecules carbohydrates
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
ZURICH
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.