Novel molecular tools for the analysis of the cell membrane proteome and interactome

Projektinformationen

MOLTOOL / INTERACTOM

ID Finanzhilfevereinbarung: 514946

Startdatum 6 Dezember 2004

Enddatum 5 Dezember 2006

Finanziert unter

• Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006

Gesamtkosten € 0,00

EU-Beitrag € 149 155,00

Koordiniert durch

UNIVERSITAET BIELEFELD
Germany

Ziel

The vast amount of data originating from the different genome initiatives in the context of systems biology require more and more emphasis to be placed on the elucidation of the different levels of dynamic processes present in the living cell. Beyond the transcription level (transcriptome), systematic analysis of the proteins expressed by a cell at a certain time and under certain conditions (proteome) is of eminent importance, as they are the major players in biological processes. Despite the fact, that many technological advances are currently involved in proteome analysis, there is still a great need for the development of novel molecular tools that permit or facilitate genome-wide proteome analysis or that allow for the tailored creation and analysis of proteome subsets.

Retrieval of biomolecular interaction partners from the proteome results in the "interactome" of a class of proteins or other biomolecules. The scientific goal of the training project is the development and application of novel engineered chemical probes for proteomics and interactomics. Focus is placed on the interaction of integrins and matrix metalloproteinases with each other and with other proteins, as both are potential target in tumour therapy. Small-molecule integrin ligands (RGD peptides) and matrix metalloproteinases (hydroxamate inhibitors), respectively, will be chemically modified to allow for the immobilisation of the ligands to magnetic beads or affinity chromatography materials. Proteins will be detected together with their native interaction partners because of the non-denaturing conditions.

These new developments will play a major role on the contact points between chemical biology and systems biology, one of the biggest challenges of the next decades. The combination of promising and feasible concepts, highly relevant targets, cutting edge technology and interdisciplinary research will bring optimum benefit with respect to training issues for the candidate.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › proteins › proteomics
• natural sciences › chemical sciences › organic chemistry
• medical and health sciences › clinical medicine › obstetrics
• natural sciences › biological sciences › genetics › genomes

Schlüsselbegriffe

• Inetraction analysis of biomolecules
• Interactomics
• affinity chromatography
• immobilization
• integrins
• magnetic beads
• matrix metalloproteinases
• protein ligands

Programm/Programme

• FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006

Thema/Themen

• MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF)

Aufforderung zur Vorschlagseinreichung

FP6-2002-MOBILITY-5
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

EIF - Marie Curie actions-Intra-European Fellowships

Koordinator

UNIVERSITAET BIELEFELD

• EU-Beitrag: Keine Daten
• Adresse: BIELEFELD ; Deutschland
• Gesamtkosten: Keine Daten