Objective
The epithelial sodium channel (ENaC) is assumed to play a major role in the pathogenesis of chronic lung disease incystic fibrosis patients. Its natural regulation by the cystic fibrosis transmembrane conductance regulator (CFTR)appears to be compromised based on the impaired function of CFTR. The missing downregulation of the channel results in increased absorption of sodium ions and fluid across airway epithelia leading to the depletion of the peculiarly liquid layer and to the depression of mucus clearance. Several observations suggest that a downregulation of Ancestors the perciliary liquid layer, thereby rehydrating the mucus and improving ciliary clearance in the lung. Therefore we propose to specifically downregulate ENaC expression by RNA interference. For this purpose we develop and apply new means of nucleic acid precision targeting both on the molecular and macroscopic level. The first level of precisionist introduced by the use of ENaC-specific siRNA, a technology known for its high downregulating specificity. The second level of precision is brought about by uPA-receptor binding and nuclear localization peptide modules of novel nonviral molecular constructs for nucleic acid delivery. Protein transduction domain peptides will be used for the delivery of synthetic siRNA. A level of loco-regional precision is contributed by the administration of such constructs via the airwaysupon aerosolization. Yet another and novel level of precision and targeting is introduced by the association of viral and nonviral constructs for siRNA delivery and/or expression with magnetic nanoparticles, such that lung-specific accumulation and retention can be mediated by external magnetic fields. For this purpose novel magnetic vector formulations as well as magnetic field generating equipment are developed. These novel constructs and technologies will be evaluated in fetal and postnatal animal models in order to demonstrate their efficacy.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences chemical sciences inorganic chemistry alkali metals
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences genetics RNA
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2003-LIFESCIHEALTH-I
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
MUENCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.